摘要
Abstract
Objective To investigate the changes of proliferation and invasion of glioma U251 cells transfected with vascular endothelial growth factor (VEGF) siRNA (VEGF-siRNA) and its mechanism.Methods Human glioma U251 cells were divided into the observation group, control group, and blank group which were transfected with VEGF-siRNA, siRNA-NC, and empty plasmid for 48 h, respectively.CCK-8 method was used to observe the proliferation of cells in each group (expressed in OD490), Transwell chamber was used to observe the invasion ability of each group (expressed as a number of transmembrane cells), Western blotting was used to detect the protein expression of matrix metalloproteinase 2 (MMP-2), matrix metalloproteinase 9 (MMP-9), protein kinase B (AKT) and phosphorylated protein kinase B (p-AKT).Results At 48 h after transfection, the observation group had significantly lower VEGF protein expression levels, OD490 and smaller number of transmembrane cells than the control group and blank group (VEGF protein expression: 0.161±0.040 vs 0.649±0.032 and 0.656±0.026, OD490: 0.226±0.018 vs 0.449±0.035 and 0.454±0.038, the number of transmembrane cells: 100.61±16.90 vs 284.11±13.1 and 286.18±12.24;all P<0.01).The expression of MMP-2, MMP-9 and p-AKT in the observation group was lower than that of the control group and the blank group at 48 h after transfection (all P <0.01).No significant difference was found in the expression of AKT between the observation group and the control group, the blank group, all P>0.01.Conclusion The proliferation and invasion abilities of glioma U251 cells transfected with VEGF-siRNA decrease by inhibiting the expression of MMP-2, MMP-9, AKT, and p-AKT.关键词
微小RNA/血管内皮生长因子/脑胶质瘤/细胞增殖/肿瘤侵袭/基质金属蛋白酶/蛋白激酶BKey words
microRNA/vascular endothelial growth factor/glioma/cell proliferation/tumor invasion/matrix metalloproteinase/protein kinase B分类
医药卫生
