世界中医药2017，Vol.12Issue(5)：1094-1100,1104,8.DOI:10.3969/j.issn.1673-7202.2017.05.036
艾灸对亚急性衰老大鼠胸腺CD4+T细胞IL-2、IL-2Rα、NF-κB和I-κB蛋白表达的影响
Effects of Moxibustion on the Expression of IL-2, IL-2Rα, NF-κB and I-κB in Thymus CD4+T Cells of Subacute Aging Rats
摘要
Abstract
Objective:To observe the effect of moxibustion on CD4+T cells of thymus in subacute aging rats by recording the changes of IL-2 and related receptor protein levels.Methods:Use D-galactose induced subacute aging rat model.Twenty-four male SD rats were randomly divided into normal group, model group and moxibustion group.In moxibustion group, use mild moxibustion on Shenshu Point(BL23) bilateral for 40 days, 5 minutes each day each time.To separate CD4+T cells by MACS method, and access the expression of IL-2, IL-2Rα, NF-κB and I-κB in CD4+T cell by Western Blot.Results:Western Blot assay shows that comparing to the control group, the expression of IL-2, IL-2Rα, NF-κB and I-κB in CD4+T cell of model group significantly decreased (P<0.05).However, in the moxibustion group, those increased with significant difference (P<0.05).Conclusion:In subacute aging rats, moxibustion on BL23 can improve the expression of IL-2, IL-2Rα, NF-κB and I-κB in thymic CD4+T cell.关键词
亚急性衰老/IL-2/IL-2Rα/NF-κB/I-κB/艾灸Key words
Subacute aging/IL-2/IL-2Rα/NF-κB/I-κB/Moxibustion分类
医药卫生引用本文复制引用
崔云华,沈文宾,吴焕淦,周次利,雷菲,黄立师,赵琛,李璟..艾灸对亚急性衰老大鼠胸腺CD4+T细胞IL-2、IL-2Rα、NF-κB和I-κB蛋白表达的影响[J].世界中医药,2017,12(5):1094-1100,1104,8.基金项目
国家重点基础研究发展计划("973"计划)资助项目(编号:2009CB522900 （"973"计划）
2015CB554501) （）
国家自然科学基金项目(编号:81303029 （）
81373752) （）
