世界中医药2017，Vol.12Issue(5)：1105-1108,4.DOI:10.3969/j.issn.1673-7202.2017.05.038
MTT法检测通络止痛方对人膝骨关节炎滑膜成纤维细胞增殖的影响
Effect of Tongluo Zhitong Decoction on Proliferation of Synovial Fibroblasts in Patients with Knee Osteoarthritis by MTT
摘要
Abstract
Objective:To explore the effect of Tongluo Zhitong Decoction on the proliferation of knee osteoarthritis (KOA) synovial fibroblasts.Methods:The abandoned synovial tissues were taken from patients who had knee replacement surgery in the hospital.The tissues were primarily cultured after digestion in I type collagenase.Three to four generation fibroblasts were taken for high-medium-low concentration intervention.The proliferation of KOA synovial cells was detected by 4-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and the cell morphology was observed under the inverted microscope.Results:Comparisons between the drug groups and comparisons between the drug groups and the control group,the difference was statistically significant (P<0.05).MTT assay showed that s the light OD number of KOA synovial cells became greatest in the wavelength of 490 nm when Tongluo Zhitong Decoction concentration decreased.The inhibition rates of high-medium concentration groups were positive.With the increase of the concentration,the inhibition rate increased and the inhibition rate of the low concentration group was negative.With the concentration decreased,the inhibition rate reduced.Conclusion:The medium-high concentrated Tongluo Zhitong Decoction may inhibit the proliferation of synovial cells,while low concentrated Tongluo Zhitong Decoction may promote the proliferation of synovial cells.关键词
通络止痛方/MTT法检测/人膝骨关节炎/成纤维细胞Key words
Tongluo Zhitong Decoction/MTT method/Knee osteoarthritis/Fibroblast分类
医药卫生引用本文复制引用
杨黎黎,郭玉茹,王庆甫,王欢,张栋,王伟利,丁昊彬,甘稳,徐铭康,杜汪洋..MTT法检测通络止痛方对人膝骨关节炎滑膜成纤维细胞增殖的影响[J].世界中医药,2017,12(5):1105-1108,4.基金项目
国家自然科学基金面上项目(编号:81373662) （编号:81373662）
北京中医药大学自主选题项目(编号:2016-JYB-XS192) （编号:2016-JYB-XS192）
