世界中医药2017，Vol.12Issue(5)：1109-1113,5.DOI:10.3969/j.issn.1673-7202.2017.05.039
黄芪糖蛋白对胶原诱导性关节炎小鼠脾组织T-bet及GATA-3表达的影响
Effect of Astragalus Glycoproteins on T-bet and GATA-3 Expression in Mice Spleen with Collagen-induced Arthritis
摘要
Abstract
Objective:To explore the effects of Astragalus glycoproteins on collagen-induced arthritis mice′s specific transcription factors, T-bet and GATA-3, expression, and to clarify the immune mechanism of Astragalus glycoprotein on the treatment in collagen-induced arthritis mice.Methods:CIA model was induced by bovine Ⅱcollagen in mice.Mice were randomly divided into a CIA model group, a hydrocortisone positive control group, a Astragalus glycoprotein low dose group, a middle dose group and a high dose group.HE was used to observe the CIA mice spleen tissues damage.IFN-γ and IL-4 level were measured with flow cytometry in CIA mice.Western blot was used to analyze T-bet and GATA-3 protein expression.Results:Compared with the model group, Astragalus glycoprotein improved spleen tissue damage in CIA mice, and inhibited the infiltration of inflammatory cells.Astragalus glycoprotein reduced IFN-γand IL-4 level (P<0.05) and T-bet and GATA-3 protein expression (P<0.05).Conclusion:The mechanisms of action of Astragalus glycoprotein on CIA mice may be related to the decrease of T-bet and GATA-3 expression and function balance of the Th1 and Th2 cells.关键词
黄芪糖蛋白/牛Ⅱ型胶原诱导性关节炎小鼠/GATA-3/T-betKey words
Astragalus glycoprotein/CIA/GATA-3/T-bet分类
医药卫生引用本文复制引用
张娜,赵俊云,薛慧清,刘慧,李彩彩,杨向竹,周然..黄芪糖蛋白对胶原诱导性关节炎小鼠脾组织T-bet及GATA-3表达的影响[J].世界中医药,2017,12(5):1109-1113,5.基金项目
国际科技合作专项(编号:2013DFA30700) （编号:2013DFA30700）
