世界中医药2017，Vol.12Issue(5)：1120-1123,4.DOI:10.3969/j.issn.1673-7202.2017.05.041
针刀干预对帕金森病模型大鼠小胶质细胞及IL-1β表达的影响
Effects of Acupotomy on Microglic and the Expression of IL-1B in Parkinson′s Diseases
摘要
Abstract
Objective: To explore the mechanism of acpotomy in Parkinson′s disease (PD).Methods: Fourty-eight rats were randomly divided into a model group, a normal group, an acupuncture group and an acupotomy group.Using the technology of Stereo-positioning to inject the 6-hydroxy dopamine (DAOH) into the right striatum of rats to build PD rats model;Rats in the acupotomy group were treated by disposable acupotomy at suboccipital muscle group and transverse process of C1-2, twice a week for continued four weeks.Electroacupuncture (EA) group were treated by acupuncture at Baihui and Taiyang, to strike Taiyang from Baihui, third a week for four weeks.HE staining method was used for observation of morphology, quantity of neurons and quantity of microglia, and Elisa method was used for testing the expression of IL-1β.Results: Compared with the nomal group, the quantity of the dopaminegic neurons in the damaged side of PD model group obviously decreased, while microglic increased, and the level of IL-1β was improved with significant difference(P<0.05).Compared with the model group, the quantity of the dopaminegic neurons in the damaged side of the acpotomy and EA group increased obviously, while microglic decreased, and the level of IL-1β was alleviated with significant difference(P<0.05).Conclusion: Acupotomy can improve the morphology and quantity of dopaminergic neurons and its mechanism may be mediated by inhibition of microglia activation of inflammatory reaction, reducinf the content of inflammatory cytokines IL-1 beta, thus to treat PD.关键词
帕金森病/针刀/6-羟基多巴胺(6-DAOH)/白介素-1Key words
Parkinson′s disease/Acpotomy/6-hydroxy dopamine/IL-1β分类
医药卫生引用本文复制引用
马薇薇,郭长青,芦娟,杜宁宇,梁靖蓉,吴桐,卢胜春..针刀干预对帕金森病模型大鼠小胶质细胞及IL-1β表达的影响[J].世界中医药,2017,12(5):1120-1123,4.基金项目
企事业单位委托科技项目(编号:535104032)——针刀治疗帕金森病机制的研究 （编号:535104032）
