世界中医药2017，Vol.12Issue(5)：1124-1127,4.DOI:10.3969/j.issn.1673-7202.2017.05.042
立论于肝脾同治研究逍遥散对老年性痴呆模型鼠行为学及神经可塑性的影响
Effect of Xiaoyao Powder on Praxiology and Neural Plasticity in Rats with Alzheimer′s Diseases from Simultaneous Treatment in Liver and Spleen Theory
摘要
Abstract
Objective: To study the effect of Xiaoyao Powder on praxiology and neural plasticity of growth protein (GAP-43) and synaptophysin (SYN) in rats with alzheimer′s diseases from simultaneous treatment in liver and spleen theory.Methods: Seventy-two healthy male SPF rats were randomly divided into 3 groups: sham operation group, dementia group and Xiaoyao Powder group, with 24 rats in each group.Rats in the dementia group and Xiaoyao Powder group had operation for senile dementia in AD model.Sham operation group received conventional feeding of grade SPF;dementia group was additionally treated with 0.2 mL/10 g sterile saline, while Xiaoyao Powder group received Xiaoyao Powder based on the blank group treatment and was given by 0.2 mL/10 g decoction lavage, once a day for 6 weeks.Y maze was applied for the study of learing and memorial behavior, and synaptic structure in hippocampal CA1 region was detected by transmission electron microscopy (TEM), immunohistochemistry was used to detect the expression of hippocampus CA1 growth protein (GAP-43), and Western blot (Western Blot) was applied to detect the expression of synaptophysin (SYN).Results: 1) Compared with the sham operation group, EN of the dementia group and Xiaoyao Powder group was increased (P<0.05) and CN decreased (P<0.05).EN in the Xiaoyao Powder group was lower than that of dementia group (P<0.05) and CN was higher than that in senile dementia group (P<0.05).2) The structure and morphology of the synapses were observed in the sham operated group.The structure and morphology of CA1 in the hippocampus of the Xiaoyao Powder group were significantly lower than those in the dementia group (P<0.05).3) Compared with sham operation group, the expression of GAP-43 and SYN in the dementia group and the Xiaoyao Powder group were all down-regulated (P<0.05), while the expression of GAP-43 and SYN in Xiaoyao Powder group was higher than that in dementia group (P<0.05).Conclusion: Xiaoyao Powder (from simultaneous treatment for Liver and spleen theory) may effectively improve the learning and memorial behavior of senile dementia model rats and its synaptic structure form.Its mechanism may be related to the up-regulation of growth associated protein (GAP-43) immunohistochemistry 8 and synaptophysin (SYN) on neural plasticity associated protein.关键词
肝脾同治/逍遥散/老年性痴呆/行为学/神经可塑性Key words
Liver and spleen/Xiaoyao Powder/Alzheimer′s disease/Praxiology/Neural plasticity分类
医药卫生引用本文复制引用
李琼锋,杜义斌,赵义..立论于肝脾同治研究逍遥散对老年性痴呆模型鼠行为学及神经可塑性的影响[J].世界中医药,2017,12(5):1124-1127,4.基金项目
云南省科技计划项目[编号:2015FB205(033)] （033）
