生命科学研究2017，Vol.21Issue(2)：101-105,143,6.DOI:10.16605/j.cnki.1007-7847.2017.02.002
黑曲霉中疏水蛋白基因种类分析
Analysis of Hydrophobin Genes from Aspergillus niger
摘要
Abstract
Hydrophobins play an important role in the morphology engineering of filamentous fungi.To clarify hydrophobin genes of Aspergillus niger for further research,assumed hydrophobin genes were analyzed based on the cysteine pattern and hydropathy pattern of hydrophobin amino acid sequences.Eight genes were obtained from 20 assumed hydrophobin genes based on gene sequence similarity.The proteins coded by these eight genes had the 8 cysteines pattern which all belong to class Ⅰ hydrophobin.While only two hydrophobins had hydropathy pattern of class Ⅰ,and the other 6 were considered as intermediate type of hydrophobin according to their hydropathy patterns.Finally,the similarity of amino acid sequences of these 8 hydrophobins was 0.5％.The clarification of assumed hydrophobin genes builds a solid base for hydrophobin gene cloning and knockout to confirm their function in fungal spore aggregation.关键词
黑曲霉/疏水蛋白基因/水缘性图谱/形态/孢子聚集Key words
Aspergillus niger/hydrophobin gene/hydropathy pattern/morphology/spore aggregation分类
生物科学引用本文复制引用
张燕,许可,张建国..黑曲霉中疏水蛋白基因种类分析[J].生命科学研究,2017,21(2):101-105,143,6.基金项目
国家自然科学基金资助项目(21306112) （21306112）
上海市自然科学基金资助项目(13ZR1429100) （13ZR1429100）
上海高校青年教师培养资助计划(slg14037) （slg14037）
教育部留学回国人员科研启动基金 （）
上海理工大学研究生创新基金资助项目 （）
微创励志创新基金项目(YS30808007) （YS30808007）
