生命科学研究2017，Vol.21Issue(2)：111-116,6.DOI:10.16605/j.cnki.1007-7847.2017.02.004
缺血预处理对小鼠心肌缺血再灌注损伤的保护作用研究
Effects of Ischemic Preconditioning on Myocardial Ischemia-reperfusion Injury in Mice
摘要
Abstract
To explore the effects of ischemic preconditioning on myocardial ischemia-reperfusion injury,the myocardial ischemia-perfusion model was established in C57/B6 male mice aged 6～8 weeks.Thirty-six mice were randomly divided into three groups:sham group (Sham,n=12),ischemia-reperfusion group (I/R,n=12)and ischemic preconditioning group (Ipost,n=12).The hematoxylin-eosin staining (HE),TUNEL,immunohistochemical staining,and Western-blot were used to detect the changes in myocardial tissues from three groups.Compared with the Sham group,the histopathological changes,including myocardial tissue edema and inflammatory cell infiltration,were obviously serious in the I/R group.However,Ipost group was improved significantly compared with the I/R group.TUNEL results showed that the myocardial apoptosis rate in Ipost group was significantly lower than that in I/R group (P＜0.01),though higher than that in the Sham group.In Ipost group,the nunber of capillaries on the edge of myocardial infarction area was significantly higher than that in I/R group (P＜0.01).Western-blot results indicated that the expression of PTEN protein in the myocardial tissue in Ipost group was down-regulated,whereas the AMPK phosphorylation and LC3Ⅰ/Ⅱ expressions were increased.Taken together,ischemic preconditioning may have protective effects on I/R injury,and could reduce the infarct area.The protective mechanism of Ipost may be related to reduction of myocardial cell apoptosis,down-regulation of PTEN,enhancement of the phosphorylation level of AMPK and enhancement of myocardial cell autophagy in ischemic infarcted myocardium after pretreatment.关键词
缺血再灌注损伤/缺血预处理/PTEN/凋亡/自噬/小鼠Key words
ischemia-reperfusion injury/ischemic preconditioning/phosphatase and tensin homolog deleted from chromosome 10 (PTEN)/apoptosis/autophagy/mice分类
生物科学引用本文复制引用
李佳妮,陈寄梅,朱平,赵明一,李嘉欣,颜金鹏,叶艳琼,罗玲,杨斌,詹纪春,苏其利,庄建..缺血预处理对小鼠心肌缺血再灌注损伤的保护作用研究[J].生命科学研究,2017,21(2):111-116,6.基金项目
中南大学湘雅三医院“新湘雅人才工程”资助项目(JY201524) （JY201524）
