生命科学研究2017，Vol.21Issue(2)：117-124,8.DOI:10.16605/j.cnki.1007-7847.2017.02.005
虎纹克胰素与运动干预对阻塞性黄疸肝细胞损伤修复机制的研究
The Effect of HWTX-Ⅺ and Aerobic Exercise Intervention on Repair Mechanism of Liver Injury Caused by Obstructive Jaundice in Mice
摘要
Abstract
Obstructive jaundice (OJ) is a common clinical disease.Although the obstruction could be removed by surgery,the resulting liver damage is difficult to repair.Huwentoxin-Ⅺ (HWTX-Ⅺ) is an effective trypsin inhibitor,but its potential effect on liver injury has not been explored experimentally.On the other hand,aerobic exercise has been reported to be effective in the amelioration of inflammation,and to promote the process of liver regeneration.In order to investigate the antagonistic effect of HWTX-Ⅺ treatment combined with aerobic exercise on liver injury caused by obstructive jaundice,a mouse model with the common bile duct hanging at the abdominal wall was established.In addition,treadmill training was adopted for aerobic exercise and HWTX-Ⅺ was administrated by tail vein injection.After the intervention,the morphological changes in liver were observed by HE staining and the expression levels of the inflammatory-related molecules were detected by RT-PCR and immunohistochemistry.The results showed that HWTX-Ⅺ administration combined with aerobic exercise could be beneficial to the recovery of damaged mouse liver induced by biliary obstructive jaundice.The underlying mechanism could be involved in the down-regulation of factors in the NF-κB signaling.Moreover,the joint effect of HWTX-Ⅺ treatment and aerobic exercise was much more efficient than any single-factor intervention.关键词
阻塞性黄疸(OJ)/肝损伤/虎纹克胰素(HWTX-Ⅺ)/有氧运动Key words
obstructive jaundice (OJ)/liver damage/huwentoxin-Ⅺ (HWTX-Ⅺ)/aerobic exercise分类
生物科学引用本文复制引用
周柏存,罗赤苗,陈嘉勤,毛海峰,郭音,郑核,杨华..虎纹克胰素与运动干预对阻塞性黄疸肝细胞损伤修复机制的研究[J].生命科学研究,2017,21(2):117-124,8.基金项目
湖南省卫生厅科研计划课题(B2012-155) （B2012-155）
