生命科学研究2017，Vol.21Issue(2)：130-135,6.DOI:10.16605/j.cnki.1007-7847.2017.02.007
特异性干扰ICP27基因的siRNA对HSV-1病毒复制的影响
Inhibition of HSV-1 Virus Production by siRNAs Specific for ICP27 Gene
王晓婷 1董慧 2王军 1全金梅 1张伟3
作者信息
- 1. 大连市友谊医院老年病科,中国辽宁大连116100
- 2. 大连市中心医院神经内科,中国辽宁大连116033
- 3. 大连医科大学基础医学院微生物学教研室,中国辽宁大连116044
- 折叠
摘要
Abstract
Small interfering RNAs (siRNAs) targeting ICP27 gene for herpes simplex virus 1 (HSV-1) and other highly conserved sequences for herpesviruses were designed and detected for their abilities to inhibit herpesvirus replication in vitro.Virus titer assay,real-time PCR and cytopathic effect (CPE) experiment were used to observe their inhibition effect in Vero cells challenged with HSV-1 by three small hairpin RNAs (shRNA-1,shRNA-2 and shRNA-3).The results suggested shRNA-2 (target sequence from 815 position) and shRNA-3 (target sequence from 1 367 position) could depress the replication of HSV-1.Especially,virus replication was obviously inhibited by shRNA-3 in the virus titer assay,and the inhibition ratio was 81 times comparing with Negative group.At the same time,the mRNA expression level of ICP27 gene could be decreased by shRNA-3.It indicated that shRNA-3 could effectively suppress the replication of HSV-1 and be used as a supplementary therapy for HSV-1 infectious diseases.The corresponding target sequence of shRNA-3 could therefore be used as a new target for anti-HSV-1 drugs.关键词
单纯疱疹病毒1型(HSV-1)/小干扰RNA (siRNA)/RNA干扰(RNAi)/ICP27基因Key words
herpes simplex virus 1 (HSV-1)/small interfering RNA (siRNA)/RNA interference (RNAi)/ICP27 gene分类
医药卫生引用本文复制引用
王晓婷,董慧,王军,全金梅,张伟..特异性干扰ICP27基因的siRNA对HSV-1病毒复制的影响[J].生命科学研究,2017,21(2):130-135,6.
