生命科学研究2017，Vol.21Issue(2)：136-143,8.DOI:10.16605/j.cnki.1007-7847.2017.02.008
应用LCM和蛋白质组学技术筛选肺腺癌的差异表达蛋白质
Identification of Differentially Expressed Proteins in Lung Adenocarcinoma by Laser Capture Microdissection and Proteomic Analysis
摘要
Abstract
To screen for novel proteins associated with the pathogenesis of lung adenocarcinoma (AdC),laser capture microdissection (LCM) was applied to capture and collect target cells from AdC and normal bronchial epithelium (NBE) tissues,respectively.Two-dimensional gel electrophoresis (2-DE) was performed to separate proteins of microdissected AdC and NBE.PDQuest software system was used to analyze 2-DE images,and differential protein spots between the 2 types of cells were analyzed by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS).Immunohistochemistry of tissue microarray was conducted to detect the expression of the differential protein annexin A4 in 30 cases of AdC tissues,their paracancerous lung (PCL) tissues and matched positive node metastasis (PNM) tissues.The results showed that 2-DE patterns of microdissected AdC and NBE were established by proteomic technology,and 33 differential proteins between AdC and NBE were identified,21 of which were up-regulated and 12 of which were down-regulated in AdC.What's more,immunohistochemistry showed that annexin A4 was significantly overexpressed in the AdC tissues compared with their PCL tissues,and that annexin A4 expression in matched lymph node metastases were higher than the primary tumour.The data suggest that annexin A4 might play an important role in AdC progression,and serve as a novel biomarker for lymph node metastasis in AdC.关键词
肺腺癌(AdC)/转移/膜联蛋白A4/激光捕获显微切割技术(LCM)/蛋白质组学/蛋白质筛选/双向凝胶电泳(2-DE)Key words
lung adenocarcinoma (AdC)/metastasis/annexin A4/laser capture microdissection technology (LCM)/proteomics/protein screening/two-dimensional gel electrophoresis (2-DE)分类
医药卫生引用本文复制引用
代书炎,李茂玉,李育,覃婉元,梅城,付莹,陈永恒,陈主初,彭芳..应用LCM和蛋白质组学技术筛选肺腺癌的差异表达蛋白质[J].生命科学研究,2017,21(2):136-143,8.基金项目
国家重点基础研究发展计划(2014CBA02004) （2014CBA02004）
国家自然科学基金项目(81602572) （81602572）
湖南省自然科学基金项目(2017JJ3485) （2017JJ3485）
