生态学报2017，Vol.37Issue(8)：2530-2539,10.DOI:10.5846/stxb201511162317
高通量测序及其在食物网解析中的应用进展
Progress on high-throughput sequencing and its applications in food web analysis
摘要
Abstract
High-throughput sequencing is a major breakthrough in the development of DNA sequencing technology and provides an unprecedented opportunity for modern biological scientific research,such as the DNA-based approach tracking the food chains among predators-prey or herbivores-host plants trophic interactions in ecosystems.This review illustrates and compares the principles of various types of technology,including the Roche 454,Illumina,and Ion Torrent technologies,and other recent progress.We also summarize studies that have used high-throughput sequencing technology to study interactions among generalist predators/herbivores and their prey/hosts.This review could provide new information and novel approaches to exploring molecular trophic interactions and improving our understanding of the prey/host spectrum,prey/host shift,biological control,resource allocation,conservation biology,and ecological restoration.关键词
第二代测序/捕食/植食性动物/营养关系/食物转换/宏条形码技术Key words
next generation sequencing/generalist predator/generalist herbivore/trophic interactions/diet switching/metabarcoding引用本文复制引用
王雪芹,王光华,乔飞,高其康,Kong Luen HEONG,祝增荣,程家安..高通量测序及其在食物网解析中的应用进展[J].生态学报,2017,37(8):2530-2539,10.基金项目
国家科技支撑计划项目(2012BAD19B01) （2012BAD19B01）
浙江省自然科学基金项目(LY16C140002) （LY16C140002）
科技部国家“973”基础重点研究发展规划项目(2010CB126200) （2010CB126200）
