武警医学2017，Vol.28Issue(3)：227-231,5.
18F-ML-10监测多柔比星诱发心脏毒性的可行性探讨
Feasibility of using 18F-ML-10 to monitor cardiotoxicity induced by doxorubicin
摘要
Abstract
Objective To investigate the feasibility of noninvasive monitoring of doxorubicin-induced cardiotoxicity by radionuclide apoptosis imaging agent 18F-ML-10.Methods Thirty-six KM mice were randomly and equally divided into control group,low dose (15 mg/kg) model group and high dose (20 mg/kg) model group.The mice in control group were injected with normal saline.Doxorubicin was injected into model group at some dose.After 48 hours,mice were subjected to cardiac ultrasonography and the biodistribution of 18F-FDG and 18F-ML-10 was determined with gamma counting.The mice were sacrificed to remove the heart for caspase 3 immunohistochemical detection of myocardial cell apoptosis.Results The left ventricular ejection fraction was significantly lower in low dose group (LVEF =59.49 ±5.32％) and high dose group (LVEF =52.41 ±6.47％) than that in control group (LVEF =70.58 ± 5.06％),but the uptake of 18 F-FDG in low dose group (％ ID/g =0.50 ± 0.1 1) and high dose group (％ ID/g =15.58 ± 1.92) was significantly lower than that in control group (％ ID/g =36.18 ± 3.65).The uptake of apoptotic imaging agent 18F-ML-10 in low dose group (％ ID/g =0.50 ± 0.11) and high dose group (％ ID/g =1.10 ± 0.55) was significantly higher than that in control group (％ ID/g =0.02 ± 0.02) (P ＜ 0.05).Caspase 3 immunohistochemistry showed obvious apoptosis of myocardial cells in model group,which was consistent with the uptake of 18F-ML-10 in the heart.Conclusions Apoptosis is an important pathway for doxorubicin-induced cardiotoxicity.18F-ML-10 may contribute to the detection of doxorubicin-induced myocardial apoptosis.关键词
多柔比星/心脏毒性/凋亡/18F-ML-10Key words
doxorubicin/cardiotoxicity/apoptosis/18F-ML-10分类
医药卫生引用本文复制引用
黄世明,陈薇,张锦明,马永强,岳建兰,李彦峰,林志春..18F-ML-10监测多柔比星诱发心脏毒性的可行性探讨[J].武警医学,2017,28(3):227-231,5.基金项目
天津市自然科学基金资助项目(13JCYBJC22000),武警后勤学院附属医院种子基金资助项目(FYQ201601) （13JCYBJC22000）
