现代妇产科进展2017，Vol.26Issue(5)：321-324,4.DOI:10.13283/j.cnki.xdfckjz.2017.05.001
miR-26a-5p通过靶向THAP2促进子宫内膜癌细胞凋亡
miR-26a-5p promotes the apoptosis of endometrial cancer cells via targeting THAP2
摘要
Abstract
Objective:To investigate the miR-26a-5p regulates THAP2 further influences apoptosis of endometrial cancer cells.Method:Level of miR-26a-5p in endometrial cancer cells was analyzed by real-time fluorescence quantification.miR-26a-5p overexpress and inhibit plasmids were designed.After transfecting lentivirus into endometrial cancer cells,mRNA level of miR-26a-5p and THAP2 were analyzed by real-time fluorescence quantification.The influence of endometrial cancer cells apoptosis by miR-26a-5p changes was analyzed by flow cytometry.Result:There was the expression of miR-26a-5p in endometrial cancer cell lines:Ishikawa and KLE.Lentivirus transfection changed mRNA level of miR-26a-5p obviously in endometrial cancer cells.Up regulation of miR-26a-5p can enhance mRNA level of THAP2 and accelerate apoptosis of endometrial cancer cells.Down regulation of miR-26a-5p can reduce mRNA level of THAP2 and inhibit apoptosis of endometrial cancer cells.Conclusion:miR-26a-5p can promote mRNA level of THAP2,and then induce apoptosis of endometrial cancer cells,which may play an important role in prevention,diagnose and therapy of endometrial cancers.关键词
miRNA/子宫内膜癌/THAP结构域蛋白2/凋亡Key words
MiRNA/Endometrial cancer/THAP2/Apoptosis分类
医药卫生引用本文复制引用
余娜,伍科,庄研,向江东,陈晓悦,贺银燕,万小平..miR-26a-5p通过靶向THAP2促进子宫内膜癌细胞凋亡[J].现代妇产科进展,2017,26(5):321-324,4.基金项目
国家自然科学基金青年项目(No:81001154/H1621) （No:81001154/H1621）
