现代妇产科进展2017，Vol.26Issue(5)：337-341,5.DOI:10.13283/j.cnki.xdfckjz.2017.05.005
CCL28通过增强MMP-2活力促进滋养细胞浸润功能
The chemokine CCL28 enhances human trophoblast cell invasion by promoting MMP-2 activity
摘要
Abstract
Objective:To investigate the effect of chemokine CCL28 on trophoblast cells invasion.Methods:Decidual stromal cells(DSCs) were isolated,and indentified from healthy women of early pregnancy.Protein expression of CCL28 was detected in DSCs,HTR8/Svneo cells and their co-culture supernatants by enzyme-linked immunosobent assay.The effect of rhCCL28 on HTR8/Svneo cells proliferation was assessed by the MTT assay.The flow cytometry was used to detect the effect of rhCCL28 on HTR8/Svneo cells apoptosis using annexinV-FITC/PI staining.The effect of rhCCL28 on HTR8/Svneo cells invasion was detected by Transwell chamber.MMP-2 and MMP-9 activity in the supernatants of HTR8/Svneo cells was treated by rhCCL28 in the invasion assay detected by RT-PCR and gelatin zymography.Results:The DSCs and HTR8/Svneo cells could secretion CLL28 inceased with time,and their co-culthure could promote the secreted of CCL28 greatly(P<0.01).RhCCL28 did not affect the proliferation(P>0.05) or apoptosis of HTR8/Svneo cells(P>0.05) but promoted its invasion(P<0.01) in a proper concentration through binding with its CCR10 receptotor mainly.RhCCL28 promoted cell invasion by enhancing MMP-2 activity.Conclusion:CCL28 could enhance trophoblast cell invasion by binding with receptor CCR10 through promoting the activity of MMP-2 of trophoblast cell.关键词
CCL28/滋养细胞/蜕膜基质细胞/浸润/MMP-2Key words
Chemokine/CCL28/Trophoblast cell/Invasion/MMP-2分类
医药卫生引用本文复制引用
康玲,彭诗元,秦源,李梦苑,尹国武,朱晓明..CCL28通过增强MMP-2活力促进滋养细胞浸润功能[J].现代妇产科进展,2017,26(5):337-341,5.基金项目
国家自然科学基金(No:81471474) （No:81471474）
唐都医院院创新基金(No:2015LCYJ013) （No:2015LCYJ013）
