现代妇产科进展2017，Vol.26Issue(5)：349-352,4.DOI:10.13283/j.cnki.xdfckjz.2017.05.008
半乳糖凝集素-1在子宫内膜异位症大鼠模型异位及在位内膜中的表达
Expression of galectin-1 in ectopic and eutopic endometrium of a rat endometriosis model
摘要
Abstract
Objective:To investigate the expression of galectin-1 in the ectopic and eutopic tissues of a rat endometriosis model.Methods:Twelve female Wistar albino rats were used to create models of endometriosis by autotransplantation of the uterine tissue to the ectopic sites on the peritoneum.After 4 weeks of observation,the histopathological findings of the implants and the eutopic endometrial from the same rat were observed.The immunochemistry for galectin-1 was evaluated.QRT-PCR and Western blot were performed to assess the mRNA and protein expression of gal-1.Results:Grossly,the implants grew into hemispheric cysts with neovascularization on the surface and clear or choclate-like fluid in the pouch.Microscopicly,they were confirmed to be the ectopic endometrium.The successful rate was 83.33%.Gal-1 was selectively expressed in stromal and endothelial cells of ectopic and eutopic tissues.The expression of gal-1 was higher in ectopic endometrium on both mRNA (P<0.05) and protein level(P<0.05).Conclusions:Our data suggest gal-1 has a higher level in ectopic endometrial compared to the eutopic tissues in the rat model,which provides an effective way to study the relationship between the protein and EMs.关键词
半乳糖凝集素-1/子宫内膜异位症/动物模型Key words
Galecitn-1/Endometriosis/Animal model分类
医药卫生引用本文复制引用
李子扬,金平..半乳糖凝集素-1在子宫内膜异位症大鼠模型异位及在位内膜中的表达[J].现代妇产科进展,2017,26(5):349-352,4.基金项目
深圳市科创委资助项目(No:JCYJ20150402090412995) （No:JCYJ20150402090412995）
深圳市卫计委项目(No:201501034) （No:201501034）
