摘要
Abstract
Objective:To investigate the miR-210 expression in villus tissue of patients with unexplained recurrent spontaneous abortion (URSA) and its relationship with angiogenesis factors.Methods:53 cases of patients with URSA were selected from Mar.2014 to Apr.2016,40 cases of normal early pregnant women receiving artificial abortion were selected as control group.The expressions of miR-210,HIF-1α and VEGF in villus tissue were detected by using real-time fluorescence quantitative PCR.The expressions of HIF-1α protein and VEGF protein were detected by using Western blot.The micro lymphatic vessel density(MVD) in villus tissue was measured by immunohistochemical staining.Results:The relative expression levels of miR-210,HIF-1α mRNA and VEGF mRNA in villus tissue in URSA patients were lower than those of control group,and the relative expression levels of miR-210,HIF-1α mRNA and VEGF mRNA in villus tissue in URSA patients with abortion times (>3) times were lower than URSA patients with abortion times (≤3),the differences were statistically significant (P<0.05).The relative expression levels of HIF-1α protein and VEGF protein in villus tissue in URSA patients were lower than control group,and the relative expression levels of HIF-1α protein and VEGF protein in URSA patients with abortion times (>3) were URSA patients with abortion times (≤3),the differences were statistically significant (P<0.05).The MVD value in URSA patients was (26.8±4.4),which was significantly lower than(37.2±5.1) in control group,the difference was statistically significant(t=15.394,P<0.001).Pearson correlation analysis showed that the relative expression level of miR-210 in villus tissue in URSA patients was positively correlated with the relative expression level of HIF-1α mRNA,the relative expression level of VEGF mRNA,and MVD value (r=0.418,0.507 and 0.472,P<0.05).Conclusion:The expression of miR-210 was low in villi tissues in patients with URSA,and was related with the number of abortion.It might through interaction with HIF-1α and VEGF and MVD to reduce the number of MVD,and lead URSA occur.关键词
不明原因/复发性流产/绒毛组织/miR-210/血管新生Key words
Unexplained/Recurrent abortion/Villi tissue/miR-210/Angiogenesis分类
医药卫生
