骨髓间充质干细胞分化为心肌样细胞相关基因表达与形态结构发生的关联
Cardiomyocyte-like differentiation of bone marrow mesenchymal stem cells: an association between related gene expression and cell morphological changes
摘要
Abstract
BACKGROUND:Bone marrow mesenchymal stem cells (BMSCs) have been induced to differentiate into cardiomyocyte-like cells in vitro.OBJECTIVE:To explore the association between GATA-4, Nkx-2.5 and α-myosin heavy chain (α-MHC) expression and cell morphological changes and structure formation in the process of BMSCs differentiation into cardiomyocyte-like cells.METHODS:By using myocardial lysate, BMSCs were induced to differentiate into cardiomyocytes.Immunocytochemistry staining was used to detect cardiac troponin T (cTnT) and connexin43, for the identification of cardiomyocytes. In the process of directional differentiation, RT-PCR was used to detect the expression of GATA-4,Nkx2.5 and α-MHC.RESULTS AND CONCLUSION:During the directional differentiation of BMSCs, the cells were changed from long fusiform to short rod, forming protrusions that were interconnected to form mesh-like, bamboo-like or myotube-like structure. When the cells were interconnected like a bamboo, cTNT and connexin43 positive cells were visible, and then the number of positive cells increased with the presence of myotube-like structure. RT-PCR results showed that during the induced directional differentiation of BMSCs, GATA-4, Nkx2.5 and α-MHC mRNA levels increased continuously. When interconnected cells formed a mesh-like structure, GATA-4 expression reached the peak and then kept a high level. When adjacent cells were fused into a myotube-like structure, α-MHC reached the peak. Additionally, the expression of Nkx2.5presented a time-dependent increase trend. Overall, during the induced differentiation of BMSCs into cardiomyocyte-like cells, the expression of cardiomyocyte specific genes, characterized by temporality and spatiality, is related to the changes of cell morphology and special structure formation.关键词
干细胞/骨髓干细胞/骨髓间充质干细胞/心肌细胞/NKx2.5/GATA-4/α-MHC/细胞形态,广西壮族自治区自然科学基金分类
医药卫生引用本文复制引用
徐亦辰,刘玲珑,赵文婧,王慧丰,韦雅淑,陈维平..骨髓间充质干细胞分化为心肌样细胞相关基因表达与形态结构发生的关联[J].中国组织工程研究,2017,21(13):1974-1979,6.基金项目
广西急诊与医学救援人才小高地·广西高校急诊医学重点实验室开放课题(GXJZ201505) （GXJZ201505）
广西医科大学青年科学基金资助项目(GXMUYSF201528) （GXMUYSF201528）
广西自然科学基金(2012GXNSFAA053126,桂科自0832157,桂科攻0592007-1G,桂科青0229009) （2012GXNSFAA053126,桂科自0832157,桂科攻0592007-1G,桂科青0229009）
广西高校人体发育与疾病研究重点实验(0102402214009C)Guangxi Emergency and Medical Rescue Talent Small Highland · Guangxi University Emergency Laboratory Key Laboratory Open Topics, No. GXJZ201505 （0102402214009C）
the Science Foundation for the Youth in the Guangxi Medical University, No. GXMUYSF201528 （）
the Natural Science Foundation of Guangxi Zhuang Autonomous Region,No. 2012GXNSFAA053126, guikezi0832157, guikegong0592007-1G, guikeqing0229009 （）
Key Experiments of Human Development and Disease Research in Guangxi Universities, No. 0102402214009C （）
