中国组织工程研究2017，Vol.21Issue(13)：1980-1985,6.DOI:10.3969/j.issn.2095-4344.2017.13.003
感觉神经递质降钙素基因相关肽对去势大鼠骨髓间充质干细胞增殖及成骨能力的影响
Calcitonin gene-related peptide, a sensory neurotransmitter, promotes proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells from ovariectomized rats
摘要
Abstract
BACKGROUND:Calcitonin gene-related peptide can promote the biological activity of osteoblasts. Here, we attempt to further study the effect of calcitonin gene-related peptide on osteogenic differentiation of bone marrow mesenchymal stem cells.OBJECTIVE:To observe the effects of exogenous calcitonin gene-related peptide on proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells from ovariectomized rats.METHODS:An ovariectomized osteoporosis model in female Sprague-Dawley rats was employed, which was testified by micro-CT. Bone marrow mesenchymal stem cells were obtained from the femur of rats undergoing sham operation and ovariectomy, and then cultured and induced in the culture medium and osteogenic medium containing different concentrations of calcitonin gene-related peptide, respectively. The cell proliferation was detected using MTT method.After 7 days of osteogenic induction, real-time RT-PCR and western blot were used to detect Runx2 and Osterix expression at mRNA and protein levels. After 21 days of osteogenic induction, the osteogenic differentiation potentials of the cells were detected using alizarin red staining.RESULTS AND CONCLUSION:The osteoporosis model was successfully established by bilateral ovariectomy in the female rats. The proliferative and osteogenic differentiation potentials of bone marrow mesenchymal stem cells were dose-dependently enhanced by the different concentrations of calcitonin gene-related peptide. To conclude, the calcitonin gene-related peptide can increase bone formation by promoting the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells directly.关键词
干细胞/骨髓干细胞/骨髓间充质干细胞/骨质疏松/成骨分化/降钙素基因相关肽/Runx2/Osterix/细胞增殖/成骨诱导/国家自然科学基金分类
医药卫生引用本文复制引用
陈杰,丁宇翔,刘彦普,马戈,刘伟..感觉神经递质降钙素基因相关肽对去势大鼠骨髓间充质干细胞增殖及成骨能力的影响[J].中国组织工程研究,2017,21(13):1980-1985,6.基金项目
国家自然科学基金(81271103) （81271103）
陕西省社发攻关课题(2012K16-10-01) （2012K16-10-01）
the National Natural Science Foundation of China, No. 81271103 （）
the Special Project of Social Development and High Technology in Shaanxi Province, No. 2012K16-10-01 （）
