锌转运蛋白1 siRNA转染骨髓间充质干细胞对肿瘤坏死因子α信号成脂通路的影响
Effect of bone marrow mesenchymal stem cells transfected by Zrt/Irt-like protein 1 on adipogenic differentiation via tumor necrosis factor alpha signaling pathway
摘要
Abstract
BACKGROUND:Previous studies have confirmed that ethanol can promote adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and up-regulate the expression of PPARγ and aP2 in the tumor necrosis factor α (TNF-α) signaling pathway. As a member of the ZIP protein family, Zrt/Irt-like protein 1 (ZIP1) is closely related to bone metabolism and osteogenic differentiation.OBJECTIVE:To study the effect of BMSCs transfected by ZIP1 on TNF-α signaling pathway in the process of adipogenic differentiation.METHODS:The BMSCs from rabbits were isolated and cultured under different concentrations of alcohol (0.03, 0.09,0.15, 0.21 mol/L), followed by transfection by ZIP1 siRNA and ZIP1 expression vector.RESULTS AND CONCLUSION:After culture in alcohol, the expression levels of aP2 and PPARγ proteins were both significantly increased (P < 0.05), and the level of triglyceride was increased in all alcohol groups except for 0.03 mol/L alcohol group (P < 0.05). After siRNA transfection, the expression levels of aP2 and PPARγ as well as the level of triglyceride were increased significantly in all the alcohol groups (P < 0.05); however, ZIP1 transfection decreased the expression levels of aP2 and PPARγ proteins (P < 0.05). To conclude, ZIP1 siRNA could promote the adipogenic differentiation of BMSCs through the activation of TNF-α signaling pathway.关键词
干细胞/骨髓干细胞/骨髓间充质干细胞/锌转运蛋白/成脂通路/股骨头坏死/三酰甘油/过氧化物酶体增殖物激活受体γ/脂肪细胞型脂肪酸结合蛋白/蛋白免疫印迹/乙醇/广西壮族自治区自然科学基金分类
医药卫生引用本文复制引用
章晓云,赵斌,黎金焕,袁振中,陈跃平,夏天,卓映宏,冯洋,蓝佼,董盼锋..锌转运蛋白1 siRNA转染骨髓间充质干细胞对肿瘤坏死因子α信号成脂通路的影响[J].中国组织工程研究,2017,21(13):1986-1991,6.基金项目
广西自然科学基金课题(2015GXNSFAA139136) （2015GXNSFAA139136）
广西高校青年教师基础能力提升项目(KY2016YB204) （KY2016YB204）
广西卫生厅重点课题(S201419-05) （S201419-05）
广西中医药大学校级科研项目(2015LX027) （2015LX027）
2016年研究生教育创新计划资助项目立项课题(YJS201650) （YJS201650）
2014年广西中医药大学校级培育学科-骨外科学建设项目[J13167(7)] （7）
the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 2015GXNSFAA139136 （）
the Basic Ability Improvement Project of Young Teachers in Guangxi Colleges and Universities, No. KY2016YB204 （）
the Major Project of Guangxi Health Department, No. S201419-05 （）
the Scientific Research Project of Guangxi University of Chinese Medicine, No. 2015LX027 （）
2016 Postgraduate Education Innovation Program, No. YJS201650 （）
2014 Discipline Construction for Orthopedic Surgery in Guangxi University of Chinese Medicine, No. J13167(7) （7）
