中国组织工程研究2017，Vol.21Issue(13)：2003-2008,6.DOI:10.3969/j.issn.2095-4344.2017.13.007
补骨脂素逆转谷胱甘肽-S-转移酶π介导的乳腺癌干细胞耐药性
Psoralen reverses glutathione-S-transferaseπ-mediated multidrug resistance in breast cancer stem cells
摘要
Abstract
BACKGROUND:Breast cancer stem cells not only lead to the occurrence of breast cancer, but also may cause breast cancer metastasis and recurrence. The relationship between stem cells and cell resistance is also gaining increasing attentions, and the focus on the stem cell treatment may result in unexpected results.OBJECTIVE:To explore the reversal effect of psoralen on glutathione-S-transferase π (GST-π) in human breast cancer MCF-7/ADR cells and its mechanism.METHODS:MCF-7/ADR cells were cultured and enriched in serum-free medium to obtain breast cancer stem cells.RT-PCR and western blot were used to detect the expression of GST-π at the levels of gene and protein in the MCF-7/ADR cells after treatment with 0, 4, 8, 12, 16 mg/L psoralen. To observe the activation of nuclear factor-κB,western blot was used. The expression of GST-π was detected by RT-PCR in 18 μmol/L SN50 group and 8 mg/L psoralen group. Cell counting kit-8 assay was used to detect the effect of doxorubicin on cell proliferation.RESULTS AND CONCLUSION:Compared with the control group, psoralen reduced the expression of GST-π at the mRNA and protein levels, and significantly inhibited the activation of nuclear factor-κB. It was suggested that psoralen could reverse the multidrug resistance of human breast cancer MCF-7/ADR stem cells by decreasing the expression level of GST-π. The mechanism may be achieved by inhibiting the nuclear factor-κB signal pathway.关键词
干细胞/肿瘤干细胞/乳腺癌/谷胱甘肽-S-转移酶π/核因子κB/特异性抑制剂/逆转剂/补骨脂素/多药耐药/国家自然科学基金分类
医药卫生引用本文复制引用
花义同,王晓红,许乘凤,程凯,孙洪光,张英哲,刘健,杨振林..补骨脂素逆转谷胱甘肽-S-转移酶π介导的乳腺癌干细胞耐药性[J].中国组织工程研究,2017,21(13):2003-2008,6.基金项目
国家自然科学基金项目(81173601,30972932) （81173601,30972932）
山东省高等学校科技计划项目(J15LL51) （J15LL51）
山东省自然科学基金项目(ZR2014HQ020) （ZR2014HQ020）
滨州医学院科研计划与科研启动基金(BY2014KYQD36) （BY2014KYQD36）
滨州市科技发展计划项目(2015ZC0301) （2015ZC0301）
the National Natural Science Foundation of China, No. 81173601, 30972932 （）
the Scientific Research Plan of Shandong Provincial High Educations, No. J15LL51 （）
the Natural Science Foundation of Shandong Province, No.ZR2014HQ020 （）
the Scientific Research Plan and Initial Fund of Binzhou Medical University, No. BY2014KYQD36 （）
the Science and Technology Development Plan of Binzhou City, No. 2015ZC0301 （）
