中国组织工程研究2017，Vol.21Issue(14)：2140-2146,7.DOI:10.3969/j.issn.2095-4344.2017.14.002
三维打印制备掺铁介孔硅酸钙/3-羟基丁酸-3-羟基己酸共聚酯复合支架
Three-dimensional printing of Fe-containing mesoporous calcium-silicate / poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) composite scaffolds
摘要
Abstract
BACKGROUND: Three-dimensional (3D) printing technique has showed unparalleled advantages in the field of tissue engineering scaffold preparation because of its outstanding merits of convenience, efficiency, controllability and ability to construct complex shapes.OBJECTIVE: To fabricate Fe-containing mesoporous calcium-silicate (MCS) /poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) composite scaffolds using the 3D printing technique and to test the characterization and cellular biocompatibility of the composite scaffolds.METHODS: Four groups of Fe-containing MCS/PHBHHx composite scaffolds were fabricated using 3D printing technique. The molar percentage of Fe in these four groups was 0%, 5%, 10%, 15%, respectively and they were marked as 0Fe-MCS/PHBHHx, 5Fe-MCS/PHBHHx, 10Fe-MCS/PHBHHx and 15Fe-MCS/PHBHHx. The scanning electron microscopy was used to observe the microstructure of the scaffolds after being soaked in the simulated body fluid.Osteoblast cell lines MC3T3-E1 were seeded on these four groups of scaffolds as well. Cell counting kit-8 method was adopted to test the cell proliferation at 1, 3, 7 days of culture. Intracellular alkaline phosphatase activity was tested at 7 and 14 days of culture.RESULTS AND CONCLUSION: (1) Compared with the scaffolds with no soaking process, spherical particles were formed on the scaffolds because of mineralization after soaking 3 days in the simulated body fluid. (2) At 1 day of culture,there was no difference in cell proliferation among the four groups. At 3 days of culture, the proliferation rate of the 15Fe-MCS/PHBHHx scaffold was remarkably higher than that of the rest three groups (P < 0.05). At 7 days of culture,the proliferation rate was significantly higher in the 10Fe-MCS/PHBHH and 15Fe-MCS/PHBHHx scaffolds than the 0Fe-MCS/PHBHH scaffold (P < 0.05), as well as significantly higher in the 15Fe-MCS/PHBHHx scaffold than the 10Fe-MCS/PHBHH scaffold (P < 0.05). (3) At 7 days of culture, no difference in alkaline phosphatase activity could be found among these four groups of scaffolds; however, at 14 days, the 5Fe-MCS/PHBHHx, 10Fe-MCS/PHBHHx and 15Fe-MCS/PHBHHx scaffolds exhibited an enhanced alkaline phosphatase activity compared with the 0Fe-MCS/PHBHHx scaffold. Meanwhile, the 15Fe-MCS/PHBHHx showed the highest alkaline phosphatase activity.These findings indicate that the MCS/PHBHH scaffolds containing Fe could promote the proliferation and osteogenic differentiation of the MC3T3-E1 cells.关键词
生物材料/骨生物材料/三维打印/生物相容性/硅酸钙/3-羟基丁酸-3-羟基己酸共聚酯/MC3T3-E1细胞/支架/CCK-8/碱性磷酸酶分类
医药卫生引用本文复制引用
张旭,吴良浩,李得见,敖荣广,陈帆成,禹宝庆..三维打印制备掺铁介孔硅酸钙/3-羟基丁酸-3-羟基己酸共聚酯复合支架[J].中国组织工程研究,2017,21(14):2140-2146,7.基金项目
上海市科委基础研究领域项目(13JC1407302) （13JC1407302）
浦东新区卫生系统重点学科群建设资助(PWZxq2014-03) （PWZxq2014-03）
上海市卫生局重点课题(20134039) Funding: The Shanghai Science and Technology Commission Foundation for Basic Research, No. 13JC1407302 （20134039）
Disciplines Group Construction Project of Pudong Health Bureau of Shanghai, No. PWZxq2014-03 （）
Major Scientific Research Projects of Shanghai Municipal Health Bureau, No. 20134039 （）
