中国组织工程研究2017，Vol.21Issue(14)：2170-2179,10.DOI:10.3969/j.issn.2095-4344.2017.14.007
聚二甲基硅氧烷基材表面修饰及力学强度变化对关节软骨细胞的影响
Biological behaviors of articular chondrocytes on the polydimethylsiloxane with differential surface modifications and stiffnesses
摘要
Abstract
BACKGROUND: Polydimethylsiloxane (PDMS) is widely used in the basic research on cell biology because of its good biocompatibility and ability to be processed at the micro/nano level. However, cell culture on PDMS has been generally compromised by its strong hydrophobicity.OBJECTIVE: To perform a comparative investigation on the influences of different surface modifications as well as stiffness of PDMS on cellular behaviors.METHODS: PDMS films with varying stiffnesses were subjected to various surface modifications, including serum incubation, type I collagen deposition and air plasma treatment. Bovine articular chondrocytes were seeded on PDMS films and cell adhesion, proliferation and matrix production were characterized using F-actin staining, cell counting kit-8,microscopic examination, Sirius red/Safranin-O staining and quantitative determination of glycosaminoglycans,respectively.RESULTS AND CONCLUSION: Serum incubation, type I collagen deposition and air plasma treatment were all found to promote adhesion and proliferation of bovine articular chondrocytes from the results of F-actin staining and cell prolifereration curve, with air plasma treatment the best. Total amount of glycosaminoglycans (GAG) secretion was only increased by air plasma treatment and GAG/DNA was decreased by all modifications. Stiffness also played roles in cell adhesion, proliferation and GAG production, which was found to be dependent on surface modifications. This study would provide guidance for applying PDMS in cell culture.关键词
生物材料/软骨生物材料/聚二甲基硅氧烷/牛关节软骨细胞/表面修饰/力学强度/血清孵育/胶原沉积/等离子体处理/细胞生长/组织学染色/糖胺聚糖/上海市自然科学基金分类
医药卫生引用本文复制引用
熊志苗,叶朝阳,周燕,谭文松..聚二甲基硅氧烷基材表面修饰及力学强度变化对关节软骨细胞的影响[J].中国组织工程研究,2017,21(14):2170-2179,10.基金项目
上海市自然科学基金项目(16ZR1408700) Funding: the Natural Science Foundation of Shanghai, No. 16ZR1408700 （16ZR1408700）
