中国组织工程研究2017，Vol.21Issue(14)：2186-2191,6.DOI:10.3969/j.issn.2095-4344.2017.14.009
纳米羟基磷灰石/大管径TiO2纳米管复合涂层促成骨
Large-diameter TiO2 nanotubes with nano-hydroxyapatite offer an improvement in bone-forming ability
摘要
Abstract
BACKGROUND: Both hydroxyapatite (HA) and large diameter TiO2 nanotubes have excellent biocompatibility, but bone-forming ability of nano-HA (nHA) deposited large diameter TiO2 nanotubes is rarely reported.OBJECTIVE: To evaluate the bone-forming ability of nHA/large-diameter TiO2 nanotube composite coating.METHODS: Large-diameter TiO2 nanotubes were prepared by anodic oxidation method, and then nHA was electrochemically deposited on the surface of TiO2 nanotubes. Preosteoblasts MC3T3-E1 were co-cultured with the nHA/large diameter TiO2 nanotube composite, pure titanium and TiO2 nanotube coatings, respectively. At 0.5, 1, 2 hours after culture, the initial cell adhesion was observed. At 1, 3, 5 day after culture, cell proliferation was assessed. At 2 days after culture, cell morphology was observed. At 3 and 7 days after osteogenic induction, intracellular alkaline phosphatase activity was detected. At 14 days after osteogenic induction, mineralization of extracellular matrix was detected.RESULTS AND CONCLUSION: (1) After 2 hours of culture, the number of adherent cells on the composite coating was significantly lower than that on the TiO2 nanotube coating (P < 0.05), but slightly higher than that on the pure titanium coating with no statistical difference. (2) After 1, 3, 5 days of culture, the cell proliferation on the composite coating was significantly lower than that on the TiO2 nanotube coating (P < 0.05), but slightly higher than that on the pure titanium with no statistical difference. (3) The cells on the pure titanium showed a spindle-shape, while those on the TiO2 nanotube coating processed filopodia. The cells on the composite coating showed polygonal shape with a larger number of filopodia. (4) The intracellular alkaline phosphatase activity of the composite coating group was significantly higher than that of the pure titanium group and TiO2 nanotube group. The trend of mineralization of extracellular matrix was ranked from high to low: the composite coating group > TiO2 nanotube group > pure titanium group. To conclude, the nHA/large diameter TiO2 nanotube composite coating not only has good biocompatibility, but also has the ideal ability to promote bone formation.关键词
生物材料/纳米材料/羟基磷灰石/钛钠米管/前成骨细胞/生物相容性/成骨/国家自然科学基金分类
医药卫生引用本文复制引用
雷则鸣,张杭州,田昂,尤俊华,史晓国,刘兴旺,魏博,白希壮..纳米羟基磷灰石/大管径TiO2纳米管复合涂层促成骨[J].中国组织工程研究,2017,21(14):2186-2191,6.基金项目
国家自然科学基金(81671811) （81671811）
国家青年科学基金(81501857) （81501857）
辽宁省省直医院改革重点临床科室诊疗能力建设项目(LNCCC-A03-2014) （LNCCC-A03-2014）
沈阳市人口与健康科技攻关专项(F15-139-9-23) Funding: the National Natural Science Foundation of China, No. 81671811 （F15-139-9-23）
the National Science Fund for the Youth, No.81501857 （）
Diagnosis and Treatment Capacity Construction for Key Clinical Departments in the Liaoning Provincial Hospital Reform, No. LNCCC-A03-2014 （）
Special Science and Technology Research Project for Population and Health in Shenyang, No. F15-139-9-23 （）
