中国组织工程研究2017，Vol.21Issue(14)：2208-2214,7.DOI:10.3969/j.issn.2095-4344.2017.14.013
改良型富血小板纤维蛋白在兔颅骨诱导成骨中的组织学观察
Histological observation on rabbit skull osteoinduction by advanced platelet-rich fibrin
摘要
Abstract
BACKGROUND: Previous studies have proved platelet-rich fibrin (PRF) with osteoinduction ability, and the centrifugal speed and time to prepare rabbit advanced PRF (A-PRF) with the most similar structure to that of human PRF have been determined.OBJECTIVE: To observe the histological changes during A-PRF-induced osteogenesis.METHODS: Thirty Japanese white rabbits were randomly divided into A-PRF and blank control groups (n=15 per group).The full-thickness defect models were established on the rabbit parietal bone, followed by implanted with A-PRF or nothing, respectively. The model rabbits were killed immediately, at 2, 4, 8 and 12 weeks after modeling, to grossly observe the bone formation, and the histological changes in the defect region were observed through hematoxylin-eosin staining, Masson staining and immunohistochemistry.RESULTS AND CONCLUSION: Unhealed defects were observed in the blank control group. Gross and histological observations showed that the speed, amount and maturity of bone formation in the A-PRF group were significantly better than those in the blank control group immediately, 4, 8 and 12 weeks after modeling (P < 0.05). Our findings suggest that the rabbit skull bone defect is successfully established. A-PRF can induce osteogenesis, and more mature newly born bones appear with time. Additionally, osteoclasts can act with osteoblasts synergically under the A-PRF induction to promote the bone formation.关键词
生物材料/骨生物材料/改良型富血小板纤维蛋白/临界性骨缺损/组织工程/骨再生/RANKL/骨保护素/骨诱导/骨替代材料分类
医药卫生引用本文复制引用
焦志立,谢晓玲,付冬梅,赵峰,孙勇..改良型富血小板纤维蛋白在兔颅骨诱导成骨中的组织学观察[J].中国组织工程研究,2017,21(14):2208-2214,7.基金项目
全军"十二五"科研面上课题(CWS11J024) Funding: the General Twelfth Five-Year Research Project of Chinese PLA, No. CWS11J024 （CWS11J024）
