中国组织工程研究2017，Vol.21Issue(14)：2227-2233,7.DOI:10.3969/j.issn.2095-4344.2017.14.016
林蛙皮胶原/壳聚糖复合海绵的制备及性质
Preparation and properties of collagen/chitosan composite sponge from forest frog skin
摘要
Abstract
BACKGROUND: Forest frog is a rare medicinal animal in China, but the skin of forest frog is waste after Oviductus Ranae production. The forest frog skin is rich of collagen, and is large in quantity without pollution and disease risk. So the forest frog skin has potential to be developed into collagen sponge; however, there is no research on collagen sponge preparation as yet.OBJECTIVE: To optimize the preparation of collagen sponge from forest frog skin, and to investigate the physical properties and in vitro cell compatibility.METHODS: Chitosan and glutaraldehyde were used to modify collagen sponge from forest frog skin. Chitosan/collagen (w/w) (1:1, 1:2, 1:4) and glutaraldehyde concentration (1%, 1.5% and 2%) were selected as the experimental factors.The significant water absorbency, mechanical properties and thermal denaturation temperature were chosen as the indexes. Using the orthogonal experimental design, we optimized collagen sponge preparation process. We also investigated the in vitro cell compatibility and surface morphology of the collagen sponge. The nine kinds of collagen sponges from forest frog skins were co-cultured with human foreskin fibroblasts to detect cell proliferation.RESULTS AND CONCLUSION: When the chitosan/collagen was 1:1 and the glutaraldehyde concentration was 1%, we could get the collagen sponge with ideal water absorbency (water absorption capacity=5.22), mechanical properties (elongation at break=10.96%) and thermal denaturation temperature (81.24 ℃). The aperture of the forest frog skin collagen sponge was 200-400 μm, and the pores were consistent in the size and arranged regularly. Except the chitosan/collagen of 1:4 and the glutaraldehyde concentration of 1% or 5%, all kinds of forest frog skin collagen sponges could promote the viability of human foreskin fibroblasts and exerted benefits to cell viability and growth. To conclude,the forest frog skin collagen sponge has good biocompatibility and apparent morphology, in aggreement with the requirements of biological materials.关键词
生物材料/材料相容性/林蛙/胶原海绵/制备工艺/物理性质/细胞相容性分类
医药卫生引用本文复制引用
卢静,王雨佳,凌炜诗,陈旻骁,李长红,关爽,邓旭明..林蛙皮胶原/壳聚糖复合海绵的制备及性质[J].中国组织工程研究,2017,21(14):2227-2233,7.基金项目
十二五"国家科技支撑计划项目(2013BAD16B09) Funding: the National Science & Technology Pillar Program during the Twelfth Five-Year Plan Period, No. 2013BAD16B09 （2013BAD16B09）
