中国组织工程研究2017，Vol.21Issue(14)：2247-2253,7.DOI:10.3969/j.issn.2095-4344.2017.14.019
丹参素涂层β-磷酸三钙支架材料的制备与细胞相容性
Preparation and cytocompatibility of beta-calcium phosphate scaffolds with tanshinol coating
摘要
Abstract
BACKGROUND: Revascularization is a challenge for the tissue-engineered bone carrying cells after implanted into human body. Previous studies have found that tanshinol can improve the functions of endothelial progenitor cells and exert vascular protective effects.OBJECTIVE: To prepare the β-calcium phosphate (β-TCP) scaffold with tanshinol coating, and to observe its cytocompatibility.METHODS: The β-TCP scaffolds coated with 10-7, 10-6 and 10-5 mol of tanshinol were constructed by negative pressure absorption method. The distribution of tanshinol coating on the scaffold was observed using scanning electron microscopy,and the inner ingredients were analyzed by infrared spectrum. Human endothelial progenitor cells (hEPCs) were cultured in the extracts of β-TCP and β-TCP scaffolds with 10-7, 10-6, 10-5 mol of tanshinol coatings, respectively. The cell proliferation was detected at 2, 4, 6, 8 and 10 days of culture; the levels of nitric oxide and vascular endothelial growth factor in the supernatants were detected at 1, 7 and 14 days of culture; the lumen formation on the matrigel was observed after 14-day culture. hEPCs were respectively seeded onto the β-TCP and β-TCP scaffolds with different dosages of tanshinol coating,and then the cell growth was observed under scanning electron microscope at 7 days.RESULTS AND CONCLUSION: The tanshinol coating evenly distributed on the inner surface of the pores, and its crystalline structure became dense with dosage increasing. Infrared spectrum analysis revealed no changes in the characteristic absorption peak of tanshinol and TCP in the scaffold. The β-TCP scaffolds with tanshinol coating could promote the proliferation of hEPCs, especially the scaffolds with 10-6 and 10-5 mol tanshinol coating. Compared with the β-TCP scaffold, the scaffolds with 10-6 and 10-5 mol tanshinol coating significantly upregulated the nitric oxide level at 14 days of culture, and significantly increased the level of vascular endothelial growth factor at 7 and 14 days of culture (P <0.05). Although it could be found in all β-TCP scaffolds with tanshinol coating, the lumen formation was the maturest in the scaffold with 10-5 mol tanshinol coating. These results suggest the β-TCP scaffolds with tanshinol coating can promote the proliferation and endothelial differentiation of hEPCs, and hold a good cytocompatibility.关键词
生物材料/材料相容性/β-磷酸三钙/丹参素/血管内皮祖细胞/细胞相容性/支架材料/国家自然科学基金分类
医药卫生引用本文复制引用
吴涛,刘英超,南开辉,舒涛,吴金辉,卢志勤,邢建洲,吕军..丹参素涂层β-磷酸三钙支架材料的制备与细胞相容性[J].中国组织工程研究,2017,21(14):2247-2253,7.基金项目
国家自然科学基金(81303107) （81303107）
广东省自然科学基金(S2012040006295) （S2012040006295）
广东省医学科研基金(A2012499, A2014499) Funding: the National Natural Science Foundation of China, No. 81303107 （A2012499, A2014499）
the Natural Science Foundation of Guangdong Province, No. S2012040006295 （）
the Medical Research Foundation of Guangdong Province, No. A2012499 and A2014499 （）
