畜牧与兽医2017，Vol.49Issue(5)：1-7,7.
湖羊和巴什拜羊FSHR基因多态性分析
Polymorphism of FSHR genes in Hu sheep and Bashibai sheep
摘要
Abstract
This study was performed to investigate the sequence characteristics of 3'-untranslated region (3'-UTR) of follicle stimulating hormone receptor (FSHR) in ovine (Hu sheep and Bashibai sheep).We analyzed the single nucleotide polymorphism (SNP) in FSHR gene between the different litter size of Hu sheep and Bashibai sheep so as to explore its mechanism.The 3'-UTR sequences of FSHR gene were obtained using PCR,cloning and sequencing technology and we used the bioinformatics methods to analyze its sequence characterization in the Hu sheep and Bashibai sheep.The results indicated that 788 bp of 3'-UTR sequence was obtained in Hu sheep and Bashibai sheep,and Hu sheep shared similarities of 98.17％ to Bashibai sheep.Both of them existed poly A signal,ARE conservative elements and multiple miRNA binding sites.We found a T nucleotide's insertion / deletion at the stop codon 325nt by DNA pool technology,which named * 325delT.There were three genotypes (TT,T-and--) at * 325delT site in the 3'-UTR of FSHR gene in Hu sheep and Bashibai sheep.The T (0.604) was the dominant allele in Hu sheep.However,the-(0.635) was the dominant allele in Bashibai sheep.Luciferase activity analysis showed that the mutation of * 325delT had no significant effect on the transcriptional activity of ovine FSHR gene.We obtained the sequence of 3'-UTR in ovine FSHR gene and identified a new SNP,and the polymorphism of * 325delT in FSHR might be association with litter size in sheep.关键词
湖羊/巴什拜羊/FSHR/SNP/多态性Key words
Hu sheep/Bashibai sheep/FSHR/SNPs/polymorphism分类
农业科技引用本文复制引用
王德迪,安外尔·热合曼,姚一龙,潘增祥,决肯·阿尼瓦什,李齐发..湖羊和巴什拜羊FSHR基因多态性分析[J].畜牧与兽医,2017,49(5):1-7,7.基金项目
江苏省自然科学基金(BK20141369)和江苏省农业科技自主创新资金项目[CX (15) 1007] （BK20141369）
