畜牧与兽医2017，Vol.49Issue(5)：58-62,5.
不同冷冻载体对牛未成熟卵母细胞玻璃化冷冻效果的影响
Effect of different cryopreservative carriers on vitrification of bovine immature oocytes
摘要
Abstract
The aim of this study was to explore the fabrication methods of different cryopreservative carriers and their effect on vitrification of bovine immature oocytes.Firstly,open pulled straw (OPS) and glass micropipette (GMP) were made successfully by hand.Then,bovine cumulus oocyte complexes (COCs) were vitrified using OPS,GMP and Cryoleaf as carriers respectively.After warming,the oocytes were maturated,fertilized and cultured in vitro.The rates for normal morphology,maturation,cleavage,and blastocyst were determined.Results showed that OPS could be made of straws using simple small alcohol lamp.GMP could be made of thin glass tube using alcohol burner.The normal morphology of cryoleaf group (82.1± 1.3) ％ was significantly higher than OPS (74.3-± 1.8) ％ and GMP (72.5± 2.6) ％ group (P＜0.05),however,there was no significant difference between OPS and GMP group (P＞0.05).Furthermore,there were no significant differences in the maturation rate,cleavage rate and blastocyst rate among three groups (P＞0.05).Together,bovine immature oocytes can be vitrified effectively by OPS,GMP and Cryoleaf.关键词
牛/未成熟卵母细胞/冷冻载体/玻璃化冷冻Key words
bovine/immature oocyte/cryopreservative carrier/vitrification分类
农业科技引用本文复制引用
裴旭哲,马德全,张凡,武花,郭先飞,李晓霞,栗颖华,禹学礼..不同冷冻载体对牛未成熟卵母细胞玻璃化冷冻效果的影响[J].畜牧与兽医,2017,49(5):58-62,5.基金项目
国家自然科学基金(31172207和31201801) （31172207和31201801）
河南省教育厅科学技术研究重点项目(14A230015) （14A230015）
