烟草科技2017，Vol.50Issue(4)：10-17,8.DOI:10.16135/j.issn1002-0861.2016.0436
不同产地和部位对片烟自然醇化过程中细菌群落结构的影响
Effects of growing area and stalk position on bacterial community structure in tobacco strips during aging
摘要
Abstract
To investigate the differences of bacterial community structure among tobacco strips from different growing areas and stalk positions during aging, the bacterial 16S rDNA of strip samples of upper, middle and lower leaves from 4 areas (Youxi in Fujian, Zhanyi in Yunnan, Changshun in Guizhou and Queshan in Henan) during aging were sequenced by Illumina MiSeq. The results showed that 940 operational taxonomic units (OTUs) were obtained from 12 samples. The samples (C3F) from Youxi had the most abundant bacterial biodiversity. The dominant phyla in 12 samples were Proteobacteria, and the dominant genera in most samples were Sphingomonas. The Venn diagram of bacterial OTUs of strips from different areas during aging revealed that 195 of OTUs were common to the strips from 4 areas, the numbers of specific OTUs for Youxi, Zhanyi, Changshun and Queshan were 300, 9, 4 and 0, respectively.关键词
片烟/自然醇化/细菌/MiSeq/16SrDNA/群落结构Key words
Tobacco strip/Aging/Bacterium/MiSeq/16S rDNA/Community structure分类
轻工纺织引用本文复制引用
包可翔,林俭,何伟,陈善义,颜奕华,李菁菁,陈义强,陈少滨,范坚强..不同产地和部位对片烟自然醇化过程中细菌群落结构的影响[J].烟草科技,2017,50(4):10-17,8.基金项目
福建中烟工业有限责任公司科技项目"四段式气调醇化过程中各个节点烤烟叶面微生物的分离鉴定"(JSZX2014020) （JSZX2014020）
中国烟草总公司面上项目"四段式烟叶醇化及综合养护技术研究"(FJZYJH2012011). （FJZYJH2012011）
