埃罗替尼对小鼠角膜上皮组织病理学及超微结构的影响
Effects of erlotinib on histopathology and ultrastructure of corneal epithelium in mice
摘要
Abstract
Objective To investigate the histopathological and ultrastructural changes of corneal epithelium induced by erlotinib in mice.Methods Totally 30 6-8 weeks old male BALB/c mice were divided into three groups:Control group (n =12),experimental group (n =12),another 6 mice did nothing as the blank control.Experimental group used erlotinib eye drops and control group used PBS in both eyes,four times per day.At 1 day,7 days and 14 days after the intervention,corneal fluorescence staining (FL) was observed by slit lamp and graded.On the fourteenth day after the intervention,the eye balls of mice were taken,and the histopathological and ultrastructural changes of corneal epithelium and epithelial cells were observed by optical microscope and electron microscope,respectively.And protein of cornea was measured by Western Blot.Results Before the intervention,there was no significant difference in FL scores between the experimental group and control group (P ＞ 0.05).At 1 day,7 days and 14 days,FL score of experimental group was significantly higher than the groups of non-intervention,the difference was statistically significant (all P ＜ 0.05).While FL score of control group was not statistically significant before and after intervention (all P ＞ 0.05);Compared between two groups,there were statistical differences at 7 days,14 days in FL score (all P ＜ 0.05).In the experimental group,the histopathological changes of murine corneal epithelial cells had disorderly arrangement,increased layers of cells,and the inflammatory cells.Under electron microscope,the morphology of corneal epithelial surface cells was irregular and partially detached.The number of microvilli,desmosomes and hemidesmosomes were significantly decreased when compared to the control group.The expression of p-EGFR in experimental group was significantly less than that in control group,the difference was statistically significant (P ＜ 0.05).Conclusion Erlotinib can damage the tissue structure of corneal epithelium and ultrastructure of corneal epithelial cells in mice.And the mechanism is probably that erlotinib influence the corneal epithelium by inhibiting the EGFR activation.关键词
埃罗替尼/角膜上皮/电子显微镜/微绒毛/超微结构Key words
erlotinib/corneal epithelium/electron microscope/microvillus/ultrastructure分类
医药卫生引用本文复制引用
罗阿丽,李娟,杨启晨,王春宝,牛静静,叶蕾,容蓉,邵毅..埃罗替尼对小鼠角膜上皮组织病理学及超微结构的影响[J].眼科新进展,2017,37(5):401-405,5.基金项目
国家自然科学基金资助(编号:81660158、81160118、81400424、81460092、81400372) （编号:81660158、81160118、81400424、81460092、81400372）
江西省远航工程(编号:2014022) （编号:2014022）
江西省自然科学基金重大项目(编号:2016ACB21017) （编号:2016ACB21017）
江西省自然科学基金(编号:20151BAB215016) （编号:20151BAB215016）
江西省科技支撑计划项目(编号:20151BBG70223) （编号:20151BBG70223）
江西省卫计委面上项目(编号:20175116) （编号:20175116）
陕西省创新人才推进计划项目(2017) （2017）
陕西省科学技术研究发展计划项目(编号:2014K11-03-07-04)National Natural Science Foundation of China (No:81660158,81160118,81400424,81460092,81400372) （编号:2014K11-03-07-04）
Jiangxi Province Voyage Project (No:2014022) （No:2014022）
Natural Science Key Project of Jiangxi Province (No:2016ACB21017) （No:2016ACB21017）
Youth Science Foundation of Jiangxi Province (No:20151BAB215016) （No:20151BAB215016）
Science and Technology Foundation of Jiangxi Province(No:20151BBG70223) （No:20151BBG70223）
Health Development Planning Commission Science Foundation of Jiangxi Province(No:20175116) （No:20175116）
Innovative Talents Promotion Project of Shaanxi Province (2017) （2017）
Science and Technology Research and Development Project of Shaanxi Province(No:2014K11-03-07-04) （No:2014K11-03-07-04）
