冻干人纤维蛋白黏合剂在围绝经期雌兔干眼的应用研究
Therapetic effects of freeze-dried human fibrin glue on xerophthalmia in perimenopausal female rabbit
摘要
Abstract
Objective To explore prevention and cure effects of the freeze-dried human fibrin glue as the way of the lacrimal duct embolization on xerophthalmia in perimenopausal female rabbit.Methods A total of 72 female rabbits,after anti infection treatment and were cut off third eyelid,were made into perimenopausal xerophthalmia rabbit models.After surgery,all of these rabbits were randomly divided into 6 groups (12 rabbits per group):No treatment group after surgery (group A),PBS prevention group (group B),freeze-dried human fibrin prevention group (group C);no treatment group after modeling (modeling time:Two weeks after surgery,group D),PBS treatment group (group E),freeze-dried human fibrin treatmentgroup (group F).The Schirmer test (SIT),corneal fluorescein (FL) and corneal confocal microscope were performed before and 2 weeks,4 weeks,6 weeks after injection.Results There were statistical differences in FL score and SIT in group A,group B and group C among different time points (F =27.346,10.608;P =0.000,0.001);There were statistical differences between FL scores and SIT among three groups (F =7.579,6.786;P =0.002,0.007);There was significant difference in FL scores and SIT trends among three groups(F =44.897,3.424;P =0.000,0.045).The FL score and SIT of group D,group E and group F were significantly improved after treatment for 2 weeks,4 weeks and 6 weeks,the differences were statistically significant (t =2.906,3.654,4.504;P =0.022,0.017,0.013.t =4.573,5.759,7.231;P =0.032,0.019,0.008);The difference between FL score and SIT in group E and group F was statistically significant after treatment (t =2.776,4.124,5.324;P =0.032,0.026,0.017.t =1.969,3.122,4.324;P =0.038,0.023,0.009).After injection of 6 weeks,the epithelial basal cells (F =17.306,P =0.002) and inflammatory cells (F =34.024,P =0.000) of group A,B and C were significant changed,the differences were statistically significant.After injection of 6 weeks,the epithelial basal cells (F =3.749,P =0.042)and inflarnmatory cells(F=8.806,P =0.005) of group D,E and F were significant changed,the differences were also statistically significant.Conclusion Lacrimal duct embolization with freeze-dried human fibrin glue is effective for the xerophthalmia.关键词
冻干人纤维蛋白黏合剂/干眼症/围绝经期/雌兔Key words
freeze-dried human fibrin glue/xerophthalmia/perimenopause/female rabbit分类
医药卫生引用本文复制引用
周荃,刘康成,叶蕾,周双双,邹雪香,谭钢,姜楠,邵毅..冻干人纤维蛋白黏合剂在围绝经期雌兔干眼的应用研究[J].眼科新进展,2017,37(5):406-410,5.基金项目
国家自然科学基金资助(编号:81660158、81160118、81460092、81400372) （编号:81660158、81160118、81460092、81400372）
江西省远航工程(编号:2014022) （编号:2014022）
江西省自然科学基金重大项目(编号:2016ACB21017) （编号:2016ACB21017）
江西省自然科学基金(编号:20151 BAB215016) （编号:20151 BAB215016）
江西省科技支撑计划项目(编号:20151BBG70223) （编号:20151BBG70223）
江西省卫计委科技计划面上项目(编号:20155204、20175116)National Natural Science Foundation of China (No:81660158,81160118,81460092,81400372) （编号:20155204、20175116）
Jiangxi Province Voyage Project (No:2014022) （No:2014022）
Natural Science Key Project of Jiangxi Province (No:2016ACB21017) （No:2016ACB21017）
Natural Science Foundation of Jiangxi Province (No:20151BAB215016) （No:20151BAB215016）
Technology and Science Foundation of Jiangxi Province (No:20151BBG70223) （No:20151BBG70223）
Health Development Planning Commission Science Foundation of Jiangxi Province (No:20155204,20175116) （No:20155204,20175116）
