眼科新进展2017，Vol.37Issue(5)：411-414,4.DOI:10.13389/j.cnki.rao.2017.0104
碱性成纤维细胞生长因子(bFGF)在遗传性视网膜变性小鼠视网膜内的表达
A contrastive research on bFGF expression in retinas of rd mouse and C57BL mouse
摘要
Abstract
Objective To compare the expressions of basic fibroblast growth factor (bFGF) in retinas of rd mouse (the experiments) and C57BL mouse (the controis) of five different postnatal ages,analyze the similarities and differences of bFGF expressions,and conclude the role of bFGF during the key period that retinas developed or degenerated,Methods Eight rd mice and eight C57BL mice of postnatal 0 week,1 week,2 weeks,3 weeks,4 weeks (PN 0,1,2,3,4 W) were chosen stochastically and executed immediately,their eyeballs were excavated and made into the retinal slices,then the bFGF contents in the retinas were analyzed by immunohistochemical method,photomicroscope and semi-quantitative analysis.Results Change characteristic of the curve of bFGF contents in retina of rd mice was similar to that of C57BL mice.Both curves began to fall after born,which reached each low level at PN-1W and then rose until reaching each peak at PN-2W,at which ten layer structures of retinas in both groups were formed.Then both curves began the second fall till PN-4W.During the last two weeks,retinas of C57BL mice degenerated rapidly while retinas of rd mice continued to mature.For all the five postnatal weeks,the bFGF contents in retinas of rd mice were lower than those of C57BL mice evidently.All statistical analysis of the difference have made sense distinctly (P ＜ 0.01).Couclusion bFGF may play a protective role in the developing to maturation and sustaining of retinal structure,and the higher expression of bFGF content may prevent retinal degeneration in some extent.关键词
碱性成纤维细胞生长因子/视网膜变性/神经节细胞Key words
basic fibroblast growth factor/retinal degeneration/ganglion cell分类
医药卫生引用本文复制引用
郑浩,李根林..碱性成纤维细胞生长因子(bFGF)在遗传性视网膜变性小鼠视网膜内的表达[J].眼科新进展,2017,37(5):411-414,4.基金项目
国家自然科学基金资助(编号:81271046) （编号:81271046）
北京市自然科学基金项目(编号:KZ201510025025)National Natural Science Foundation of China (No:81271046) （编号:KZ201510025025）
Beijing Natural Science Foundation Program (No:KZ201510025025) （No:KZ201510025025）
