眼科新进展2017，Vol.37Issue(5)：415-418,4.DOI:10.13389/j.cnki.rao.2017.0105
Neu-P11对急性高眼压大鼠眼压及视网膜组织胶质原纤维酸性蛋白表达的影响
Effect of Neu-P11 on retinal GFAP protein expression and IOP of acute high IOP rats
摘要
Abstract
Objective To explore the effects of the new melatonin nonselective agonists Neu-P11 on intraocular pressure (IOP) and glial fibrillary acid protein (GFAP) expression in the retina of acute high IOP rat.Methods Twenty-four male Sprague-Dawley rats were randomly divided into 4 groups (6 cases in each group):Normal IOP with local treatment (NIL) group,high IOP with local treatment (HIL) group,HILwith melatonin treatment (HIL-M) group,HIL with Neu-P11 treatment (HIL-N) group.10 μL normal saline was instilled in NIL group and HIL group,while 10 μL 100 μmol · L-1 Mel/Neu-P11 treated in HIL-M group and HIL-N group.After 2 hours of rest,rats were placed in the Trendelenburg position duration 45 minutes.And then,IOP was measured every hour for 6 hours,and repeated it for a week.The excessive sodium pentobarbital was injected to SD rats at the end of the experiment.The rat eyeballs were took out to perform HE and immunohistochemical staining to detect retina GFAP protein expression.Results After a week,IOP in HIL group was (41.26 ± 1.73) mmHg (1 kPa =7.5 mmHg),NIL group was (13.61 ± 0.55) mmHg,which mean the Trendelenburg could induce high IOP in SD rats.Compared with the NIL group,the retinal becoming thick,the level of organization was not clear and the expression of GFAP protein was quite high in HIL group.At the same time,the GFAP protein expression and IOP were significantly weakened in HIL-M group and HIL-N group compared with HIL group.Conclusion Neu-P1 1 can reduce IOP,inhibit the activation of gliocyte,and decrease the expression of GFAP to protect the retina.关键词
Neu-P11/高眼压/胶质原纤维酸性蛋白/褪黑素/视网膜/大鼠Key words
Neu-P11/high intraocular pressure/glial fibrillary acid protein/melatonin/retina/rat分类
医药卫生引用本文复制引用
张瑶,张星慧,李美香,张弛,贺鹏程,蒋湘,尹卫东,Moshe Laudon,石金凤..Neu-P11对急性高眼压大鼠眼压及视网膜组织胶质原纤维酸性蛋白表达的影响[J].眼科新进展,2017,37(5):415-418,4.基金项目
湖南省自然科学基金(编号:14JJ2084) （编号:14JJ2084）
南华大学“蒸湘学者计划”资助(Xiangyang Tang) （Xiangyang Tang）
湖南省教育厅基金项目(编号:15C1203) （编号:15C1203）
湖南省卫生计生委基金(编号:C2015-19)Natural Science Foundation of Hunan Province (No:14JJ2084) （编号:C2015-19）
Zhengxiang Scholar Program of the University of South China (Xiangyang Tang) （Xiangyang Tang）
Fund Project of Hunan Province Department of Education(No:15C1203) （No:15C1203）
Health and Family Planning Commission of Hunan Province Fund (No:C2015-19) （No:C2015-19）
