眼科新进展2017，Vol.37Issue(5)：419-422,4.DOI:10.13389/j.cnki.rao.2017.0106
PKC活化对兔视网膜色素上皮(RPE)细胞Nrf2表达的影响
Effects of PKC activation on expression of Nrf2 in rabbit RPE cells
摘要
Abstract
Objective To investigate whether activation of protein kmase C (PKC) can induce the activation and nuclear translocation of nuclear factor enthroid 2-related factor 2 (Nrf2) in retinal pigment epithelial (RPE) cells in vitro,and explore whether PKC activation may affect the expression of Nrf2 in RPE cells.Methods PKC-specific activator phorbol ester PMA was used to culture rabbit RPE cells and RPE cells pretreated with Nrf2 inhibitor for 24 hours.Immunofluorescence and Western blot were used to detect Nrf2 in the nucleus of the expression of the situation,the data were obtained for statistical analysis.Results The expression of Nrf2 protein in the nucleus of PRE cells was detected by immunofluorescence.Compared with the control group,the expression of Nrf2 protein in the nucleus of RPE cells increased in the experimental group,and the increase of PMA + Nrf2 inhibitor group was lower than that of PMA group.The difference between the two groups was statistically significant (P ＜0.05).Western blot analysis showed that the Nrf2 protein in the nucleus of PRE was quantitatively analyzed by image analysis.The gray value of the control group was significantly different (0.286 ± 0.013 in the control group,1.304 ± 0.033 in the PMA group and 0.671 ± 0.087 in the PMA + Nrf2 inhibitor group,P ＜ 0.05).Conclusion PKC can activate nuclear translocation of Nrf2 in rabbit RPE cytoplasm,and Nrf2 inhibitor can attenuate the effect of PKC.关键词
蛋白激酶C/佛波酯(PMA)/核因子E2相关因子2/核转位Key words
protein kinase C/phorbol 12-myristate 13-acetate/nuclear factor enthroid 2-related factor 2/nuclear translocation分类
医药卫生引用本文复制引用
卫冬,教莹莹,邱璐璐,孙兆义..PKC活化对兔视网膜色素上皮(RPE)细胞Nrf2表达的影响[J].眼科新进展,2017,37(5):419-422,4.基金项目
黑龙江省卫生计生委科研课题(编号:2016-379) （编号:2016-379）
牡丹江医学院科学技术研究项目(编号:ZSSD201619) （编号:ZSSD201619）
牡丹江医学院2014年度研究生创新科研资金资助(编号:2014YJSCX-11)Heilongjiang Province Health and Life Committee Research Project(No:2016-379) （编号:2014YJSCX-11）
Mudanjiang Medical College Science and Technology Research Project (No:ZSSD201619) （No:ZSSD201619）
Mudanjiang Medical College 2014 Graduate Creative Research Fund Project (No:2014YJSCX-11) （No:2014YJSCX-11）
