金鎏 1张治国 2曹树辉 3王伟 3王旭 1程建 1苏虹 1李传友3
作者信息
- 1. 230031合肥,安徽医科大学公共卫生学院流行病与卫生统计系
- 2. 北京市昌平区结核病防治所
- 3. 首都医科大学附属北京胸科医院北京市结核病胸部肿瘤研究所细菌免疫研究室
- 折叠
摘要
Abstract
Objective To investigate distribution of interleukin-23 receptor (IL-23R) polyrnorphisms in Chinese Han and the relationship between the distribution and pulmonary tuberculosis (PTB).Methods A casecontrol study was performed,243 PTB cases were selected as case group hospitalized Beijing Chest Hospital,Capital Medical University during March 2014 to December 2015 and 226 healthy controls were recruited by the tuberculosis physical examination in Beijing Changping Center for tuberculosis control and prevention during May 2014 to October 2014.Nine polymorphic sites (rs3762318,rs11209026,rs10889657,rs10889677,rs6682925,rs1004819,rs11465804,rs2201841,rs7514847) were screened out using NCBI database,single nucleotide polymorphisms (SNPs) were detected by ligase detection reaction (LDR) for genotyping and frequency comparision,linkage analysis and haplotype analysis were performed to find out linkage disequilibrium and haplotype.Through above-mentioned methods,the association between IL-23R SNPs and PTB were estimated.Results There was a significant difference in the distribution of IL-23R gene polymorphism at polymorphic site rs6682925 between PTB cases and healthy controls,allele C frequency in cases (44.0％ (213/484)) was significantly higher than in controls (37.4％ (169/452)) (x2=4.24,P=0.040;OR=1.32,95％CI:1.01-1.71);genotype TT frequency in cases (30.1％ (73/242)) was also significantly higher than in controls (38.9％ (88/226)) (x2 =3.99,P=0.040;OR=0.68,95％CI:0.46-0.99).As to polymorphic site rs3762318,allele T frequency in cases (16.0％ (78/486))was significantly higher than in controls (10.2％ (46/450)) (x2 =7.04,P＜0.01;OR=1.69,95％CI:1.14 2.49);genotype CC frequency in cases (3.3％ (8/243)) was significantly higher than in controls (0.4％ (1/225))(x2 =5.02,P=0.040;OR=7.63,95％CI:0.95-61.46);genotype TT frequency in cases (71.5％ (173/243)was significantly higher than in controls (80.0％ (180/225)) (x2=4.89,P=0.030;OR=0.62,95％CI:0.40-0.95).Haplotype CTCACTCTG built by nine target SNPs sites in cases was found significantly different from controls by haplotype analysis (5.1％ vs.2.2％,x2=5.65,P=0.017;OR=2.44,95％CI:1.14-5.19).Conclusion Polymorphic sites rs6682925 and rs3762318 are widely existed among Chinese Han population and these two SNPs are susceptible to PTB.Haplotype CTCACTCTG built by the nine SNPs sites is a risk factor for susceptibility to PTB in Chinese Han population.关键词
结核,肺/白细胞介素23/受体,白细胞介素/多态性,单核苷酸/疾病易感性/病例对照研究Key words
Tuberculosis, pulmonary/Interleukin-23/Receptors, interleukin/Polymorphism, single nucleotide/Disease susceptibility/Case-control studies
