孙恒彪 1陈佑明 1尤旭 1潘祖汉 1肖刚 1明月2
作者信息
- 1. 南方医科大学第三附属医院,广东 广州 510630
- 2. 南方医科大学检验系,广东 广州 510630
- 折叠
摘要
Abstract
Objective To study the resistance mechanisms of carbapenem-resistant Enterobacteriaceae(CRE) to carbapenems and cephalosporins, and provide molecular evidence for the treatment of CRE infection and control of healthcare-associated infection.Methods Clinically isolated bacteria in 2014-2015 were identified and performed antimicrobial susceptibility testing by MicroScan Walkaway-40 plus system, encoding genes of carbapenems(blaNDM-1 and blaKPC-2) and encoding genes of extended-spectrum β-lactamases (blaTEM, blaSHV, blaCTX-M-1-like, blaCTX-M-2-like, blaCTX-M-8-like, and blaCTX-M-9-like)were detected, multidrug-resistance organism-related class I integron encoding gene blainT-1 was also detected.Results Seven CRE strains were isolated in 2014-2015, isolation rate was 0.30%, strain 1-6 was Klebsiella pneumoniae, strains 7 was Citrobacter freundii, all 7 strains were resistant to β-lactamase inhibitor compound,cephalosporins, cephamicins, and carbapenems,3 strains were sensitive to amikacin and tetracycline, 2 strains were sensitive to compound sulfamethoxazole.3, 2, 5, 7, 1, 4, and 5 strains of CRE were detected carrying blaNDM-1, blaKPC-2, blaTEM, blaSHV,blaCTX-M-1-like, blaCTX-M-9-like,and blainT-1 respectively, no strain was found to carry blaCTX-M-2-like and blaCTX-M-8-like genes.Conclusion blaNDM-1 and blaKPC-2 are important mechanisms leading to carbapenem resistance of 7 CRE strains;blaTEM, blaSHV, and blaCTX-M-9-like are important mechanism leading to cephalosporin resistance, blainT-1 plays an important role in multidrug-resistance to CRE and spread of multidrug-resistant genes.关键词
碳青霉烯类耐药/肠杆菌科细菌/头孢菌素/碳青霉烯酶/碳青霉烯类抗生素/多重耐药/CREKey words
carbapenem-resistant Enterobacteriaceae/Enterobacteriaceae/cephalosporin/
carbapenemase/carbapenems/multidrug resistance/CRE分类
医药卫生
