中国临床药理学杂志2017，Vol.33Issue(7)：624-627,4.DOI:10.13699/j.cnki.1001-6821.2017.07.014
毒性中药高乌头炮制前后水煎液与三氯甲烷部分对小鼠的镇痛抗炎作用
Effects of water decoction and chloroform extraction fraction before and after processing of Aconitum sinomontanum Nakaion on analgesic and anti-inflammatory in mice
摘要
Abstract
Objective To study the effects of water decoction and chloroform extraction fraction before and after processing of Aconitum sinomontanum Nakai (ASN) on the analgesic and anti-inflammatoryin mice.Methods Kunming mice were randomly divided into 10 groups (10 mice in each group)according to the weight:blank control group (0.5％ methylcellulose),positive control group (10 mg · kg-1 indomethacin + 1.6 mg · kg-1 lappaconitine),the high and low dose groups of water decoction of raw ASN (0.9,0.3 g · kg-1),the high and low dose groups of water decoction processed product (0.9,0.3 g · kg-1),the high and low dose groups of chloroform extraction fraction of ASN (4.5,1.5 mg · kg-1),the high and low dose groups of chloroform extraction fraction of processed product (4.5,1.5 mg · kg-1).Hot plate was used to study the analgesic effect of water decoction and chloroform extraction fraction before and after processing of ASN.Auricular swelling induced by dimethylbenzene in mice were observed for the evaluation of anti-inflammatory effects of it.Results Analgesic activity:Inthe hot plate test,the pain threshold of high dose groups of water decoction of processed product at 30 minafter the last administration was (24.1 ± 6.3) s,the pain threshold of high dose groups of water decoction of processed product,high and low dose groups of chloroform extraction fraction of raw product,high dose groups of chloroform extraction fraction of processed product at 120 min after the last administration were(49.7 ± 9.7),(49.8 ±7.4),(47.3 ± 6.3),(45.1 ± 7.1)s,compared with blank control group,the difference were significant (all P ＜ 0.05),respectively.Anti-inflammatory activity:The swelling degree of high dose groups of water decoction of raw product,high and low dose groups of water decoction processed product,high and low dose groups of chloroform extraction fraction of raw and processed product were (16.9 ± 11.2),(15.2 ±4.8),(17.7 ±6.4),(14.2 ±4.2),(16.0 ± 4.5),(15.7 ± 5.2),(12.5 ± 5.5) mg,compared with blank control group,the difference were significant (all P ＜0.01).The each groupof processed products compared with the each group of raw product,the difference was not significant (P ＞ 0.05).Conclusion The raw Aconitum sinomontanum Nakai have strong analgesic and anti-inflammatory effects,and processed product still remain strong analgesic and anti-inflammatory effects,and have a trend to increase compared with the raw product.关键词
高乌头/制高乌头/水煎液/三氯甲烷部分/镇痛/抗炎Key words
AconitumSinomontanumNakai/processed Aconitum Sinomontanum Nakai/water decoction/chloroform extraction fraction/analgesic activity/anti-inflammatory activity分类
医药卫生引用本文复制引用
张立军,李芸,杨志军,马骏,张艳霞..毒性中药高乌头炮制前后水煎液与三氯甲烷部分对小鼠的镇痛抗炎作用[J].中国临床药理学杂志,2017,33(7):624-627,4.基金项目
国家自然科学基金资助项目(81560650) （81560650）
