中国临床药理学杂志2017，Vol.33Issue(8)：703-705,3.DOI:10.13699/j.cnki.1001-6821.2017.08.009
银杏叶黄酮对急性脑梗死模型小鼠凋亡因子的影响
Effect of Ginkgo biloba flavone on apoptosis factor in model mice with acute cerebral infarction
摘要
Abstract
Objective To explore the effect of Ginkgo biloba flavone on the expression of hypoxia inducible factor-1 α (HIF-1 α),tumor necrosis factor-α (TNF-α) and Caspase-3 in serum of model mice with acute cerebral infarction.Methods Twenty-four female Kunming mice were selected,8 rats were randomly selected as sham operation group,and the remaining mice were established acute cerebral infarction model.Sixteen mice were successfully established the disease,and randomly divided into model group and experimental group,each group contained 8 mice.In experimental group,mice were treated by intraperitoneal injection of Ginkgo biloba flavone (20 mg · kg-1) once a day;mice in sham operation and model groups received intraperitoneal injection of physiological saline once a day,for 7 days.After experiment,all the mice were killed and extract the serum for detecting the expression level of HIF-1α,TNF-α and Caspase-3 by ELISA assay.Results The TNF-α levels of serum in the sham operation group,model group and experimental group were (5.16 ± 1.04),(33.02 ± 20.34),(22.64 ± 10.17)ng · mL-1 respectively.The serum HIF-1α levels of serum in above three groups were (508.94 ± 123.18),(735.19 ± 324.05),(626.24 ±212.76) ng· mL-1 respectively.The serum Caspase-3 levels of serum in above three groups were (6.54 ±0.24),(12.23 ± 3.45),(10.24 ± 1.58)ng · mL-1 respectively.The TNF-α,HIF-1 α and Caspase-3 expression levels of the sham operation group was significantly higher than that of model group,the difference was statistically significant (P ＜ 0.05).The TNF-α,HIF-1 α and Caspase-3 expression levels of the experimental group was significantly lower than that of model group,the difference was statistically significant (P ＜ 0.05).Conclusion Ginkgo biloba flavonoids could obviously down-regulate the expression level of HIF-1 α,TNF-α and Caspase-3.关键词
银杏叶黄酮/急性脑梗死/低氧诱导因子-1α/肿瘤坏死因子-αKey words
Ginkgo biloba flavone/acute cerebral infarction/hypoxia inducible factor-1/tumor necrosis factor-α分类
医药卫生引用本文复制引用
项薇,潘速跃,武肖娜,罗高权,邓兵梅..银杏叶黄酮对急性脑梗死模型小鼠凋亡因子的影响[J].中国临床药理学杂志,2017,33(8):703-705,3.基金项目
广东省科技计划基金资助项目(2013B012800164) （2013B012800164）
