中国临床药理学杂志2017，Vol.33Issue(8)：718-721,4.DOI:10.13699/j.cnki.1001-6821.2017.08.013
奥氮平抑制Aβ淀粉样蛋白25-35诱导PC12细胞损伤的保护作用
Protective effect of olanzapine on amyloid β25-35 induced PC12 cells injury
摘要
Abstract
Objective To explore the protective effect of olanzapine on amyloid β25-35 (Aβ25-35) induced PC12 cells injury.Methods The cells were divided into normal group,model group and low-dose,middle-dose,high-dose test groups.The model group and test group were constructed oxidative stress model with 10 μmol · L-1 Aβ25-35.The low-dose,middle-dose,high-dose test groups were given 10,30,100 μmol · L-1 olanzapine,respectively.And control and model groups were given blank media without drug.The viability of PC12 cells,cell apoptosis and activity of Caspase 3 were measured by MTF assay,Hoechst staining and spectrophotometry,respectively.The B-cell lymphoma-2 (Bcl-2),Bcl-2 associated X protein (Bax) and the activation of phosphatidylinositol 3 kinase / protein kinase B (PI3K/AKT) were measured by Western blot.Results In the control group,model group and low-dose,middle-dose,high-dose test groups,the viability of PC12 cells were (100.00 ± 9.35) ％,(58.46 ± 5.59) ％,(67.43±6.52)％,(76.31 ±7.60)％,(89.21 ±8.85)％;the activity of Caspase 3 were (1.00 ±0.12),(3.48±0.35),(2.85 ±0.28),(2.03 ±0.20),(1.34 ±0.13)U · mg-1;the expression of Bcl-2 were (0.68 ±0.06),(0.24 ±0.02),(0.38 ±0.04),(0.63 ±0.06),(0.69 ±0.07);the expression of Bax were (0.27-±0.02),(0.82 ±0.08),(0.63 ±0.06),(0.43 ±0.04),(0.30 ±0.03);the expression of PI3K were (0.89-± 0.08),(0.22 ± 0.02),(0.34 ± 0.03),(0.50-0.05),(0.54 ± 0.05);the expression of p-AKT were (2.32 ± 0.22),(0.28 ± 0.02),(0.27 ± 0.02),(1.88 ± 0.18),(2.00 ± 0.20);the expression of p-CREB were (2.79 ±0.24),(0.18 ±0.01),(0.14 ±0.01),(0.47 ±0.04),(0.50 ±0.05).Compared with the control group,the those indexes in model group had a significant difference (P ＜ 0.01);the differences on viability of PC12 cells,activity of Caspase 3,expression of Bax,Bcl-2 and PI3K in low-dose,middle-dose,high-dose test groups and model group were statistically significant (P ＜ 0.01).Also,compared with model group,the differences of p-AKT and p-CREB in middle-dose,high-dose test groups were significant (P ＜ 0.01).Conclusion Olanza-pine has a definitive protect efficacy for A325-35 induced PC12 cells injury,which might be related to activating PI3K/AKT signal pathway.关键词
奥氮平/PC12细胞/磷脂酰肌醇3激酶/蛋白激酶B信号通路Key words
olanzapine/PC12 cell/PI3K/AKT signal pathway分类
医药卫生引用本文复制引用
陈秀娟,吴海燕,李云霞,李淑洁..奥氮平抑制Aβ淀粉样蛋白25-35诱导PC12细胞损伤的保护作用[J].中国临床药理学杂志,2017,33(8):718-721,4.基金项目
山东省自然科学基金资助项目(2003C01) （2003C01）
