中国麻业科学2017，Vol.39Issue(2)：57-60,4.
亚麻AtCesA1基因过表达载体的构建
Cellulose Synthase Gene AtCesA1's Expression Vector Construction of Transforming Flax
摘要
Abstract
In order to introduce cellulose synthase gene into flax by agrobacterium mediated method,the cellulose synthase gene AtCesA1 was cloned from Arabidopsis thaliana.Sequence analysis showed that the coding region was located between the 277-3523 base,a total of 3246 bp,which presumably encoded 1082 amino acids,and the total length of cellulose synthase gene was 3912 bp.The full length AtCesA1 cDNA was subcloned into pBI1301 by synonymy mutation,constructing a higher plant expression vector pCAMBIA1301-AtCesA1.The construction of the vector was verified by enzyme digestion and PCR identification,which can lay a foundation for further research on the function of AtCesA1 gene transformation.关键词
亚麻/纤维素合成酶基因/克隆/表达载体Key words
flax/cellulose synthase gene/clone/expresstion vector分类
农业科技引用本文复制引用
刘岩..亚麻AtCesA1基因过表达载体的构建[J].中国麻业科学,2017,39(2):57-60,4.基金项目
哈尔滨市科学技术局科技创新人才(2013RFQYJ034) （2013RFQYJ034）
黑龙江省农业科技创新工程(2014QN020) （2014QN020）
国家麻类产业技术体系(CARS-19-S03) （CARS-19-S03）
