中国麻业科学2017，Vol.39Issue(2)：64-68,5.
苎麻根际土壤水浸提液对拟南芥幼苗生长的化感效应
The Allelopathy of Ramie Rhizosphere Soil Water Extract on Arabidopsis Thaliana Seedling Growth
摘要
Abstract
The seed germination experiment is used to study the different concentrations (0,1 and 2,10 mL/dish) and the different ramie varieties rhizosphere soil water extract allelopathic effect on the model plant Arabidopsis thaliana seedlings,and relative real-time quantitative PCR (QPCR) technology was applied to probe into the YUC1 (AT4G32540) gene expression related to auxin biosynthesis after treatment.Results showed that different concentration of water extracts from rhizosphere soil of ramie had certain allelopathy on the Arabidopsis thaliana,and the allelopathy enhances with the increase of concentration.The malondialdehyde (MDA) content of Arabidopsis thaliana seedlings rises with the increase in concentration of extract under the rhizosphere ZhongZhu 1 treatment.Compared with control,malondialdehyde (MDA) content of seedlings reduced in lower concentration and increased in higher concentration of extract under the rhizosphere Xiangzhu 7 treatment.The superoxide dismutase (SOD) activity decreased with the rising of treatment concentration.QPCR detection results showed that the gene expressive quantity of YUCI reduced with the increase of water extract concentration under the processing of two ramie varieties.In conclusion,with the increase of the extract concentration,ramie has an increase Allelopathy on Arabidopsis growth.关键词
苎麻/根际土壤水浸提液/拟南芥/化感效应/抗氧化酶/YUC1基因表达量Key words
ramie/rhizosphere soil water extract/Arabidopsis thaliana/allelopathic effect/antioxidant enzymes/YUC1 gene expression quantity分类
农业科技引用本文复制引用
刘楠楠,杨瑞芳,李林林,白玉超,李雪玲,崔国贤..苎麻根际土壤水浸提液对拟南芥幼苗生长的化感效应[J].中国麻业科学,2017,39(2):64-68,5.基金项目
国家麻类产业技术体系(CARS-19-E20) （CARS-19-E20）
国家自然科学基金(31471543) （31471543）
湖南省研究生科研创新(CX2015B232) （CX2015B232）
中国农业科学院科技创新工程(ASTIP-IBFC07) （ASTIP-IBFC07）
