中国水稻科学2017，Vol.31Issue(3)：238-246,9.DOI:10.16819/j.1001-7216.2017.6156
矮秆小粒水稻潇湘矮的形态学与分子遗传学分析
Morphological and Molecular Genetic Analysis of a Dwarf and Small Grain Rice Variety Xiaoxiang'ai
摘要
Abstract
[Objective]To uncover the genetic mechanism of a dwarf and small grain variety Xiaoxiang'ai (XXA), and lay an important theoretical foundation in rice dwarfing breeding.[Method]Rice variety XXA characterized as a dwarf and small grain phenotype was derived from natural mutation under breeding process. The agronomic traits of XXA were investigated. Its sensibility to phytohormone was analyzed under GA3 and BR treatments at various concentrations. Genetic analysis of XXA with different varieties and fine mapping of xxa gene from the F2 population derived from the cross between near-isogenic lines NIL(NIP) and XXA were done. Furthermore, complementary assay was performed to confirm the candidate gene.[Result]The XXA shows short spikes and compact panicles, with significant decrease in 1000-grain weight under GA3 and BR treatments, we found that XXA is partially sensitive to GA3, whereas insensitive to BR. The segregation behavior in each of the derived F2 populations between XXA and either varieties was consistent with the Mendelian monogenic ratio of 3:1. The xxa gene was finally narrowed down to a 70 kb physical region between the InDel markers F81 and F82 on chromosome 5. Within this region, eight open reading frames (ORFs) were predicted, of which, ORF5 (LOC_Os05g26890) has been annotated as a Dwarf1 gene (D1). Furthermore, sequence analysis of all the region revealed that there are only two mutation sites including one 1-bp nonsense mutation in the 5th exon and a 3-bp deletion that resulted in a lysine deletion in the 12th exon of D1. The complementary assay showed that the abnormal phenotype of XXA can be recovered.[Conclusion]The dwarf and small grain phenotype is probably associated with GA3 metabolism. The abnormal phenotype is mainly controlled by a single recessive gene. The D1 indisputably corresponds to mutation phenotype of XXA.关键词
水稻/矮秆小粒/图位克隆/机械化制种Key words
rice/dwarf and small grain/gene mapping/mechanized propagation分类
农业科技引用本文复制引用
吕育松,谢耘丰,圣忠华,邬亚文,唐绍清,胡培松,魏祥进..矮秆小粒水稻潇湘矮的形态学与分子遗传学分析[J].中国水稻科学,2017,31(3):238-246,9.基金项目
国家自然科学基金资助项目(31471472) （31471472）
国家重点研发计划资助项目(2016YFD0101801) （2016YFD0101801）
浙江省自然科学基金资助项目(LY14C30009). （LY14C30009）
