中国水稻科学2017，Vol.31Issue(3)：299-306,8.DOI:10.16819/j.1001-7216.2017.6114
八个抗稻瘟病基因在华南籼型杂交水稻中的分布
Distribution of Eight Rice Blast Resistance Genes in indica Hybrid Rice in China
摘要
Abstract
[Objective]The cloned rice blast resistant genes, Pi1, Pik-p, Pik-h, Pi2, Pi9, Piz-t, Pita and Pii, which showed broad-spectrum blast resistance in different rice growing regions, have been widely used in rice breeding for blast resistance. To clarify the distribution of these genes in hybrid rice in South China, [Method]the genotypes of resistance genes Pi1, Pik-p, Pik-h, Pi2, Pi9, Piz-t, Pita and Pii in 328 hybrid rice combinations were detected with functional markers. [Result]The distribution frequency of Pita and Pii was the highest, reaching 84.76% and 67.68% in the tested rice combinations, respectively; followed by Pi2 and Pik-p with the proportion of 22.87% and 13.72%, respectively; Pi1, Piz-t and Pik-h had the lowest distribution frequency, accounting for 5.18%, 3.35% and 2.13% of total;all of these varieties didn't carry resistance gene Pi9. The number of blast resistance genes detected in hybrid rice combinations were at most four. The resistance evaluation showed that the frequency of resistant varieties rose with increasing number of resistance genes in hybrid combinations. In a hybrid combinations with four resistance genes, the distribution frequency of resistant varieties was 91.67%. Blast resistance contribution assay of the eight resistance genes revealed that Pi2 and Pi1 displayed the greatest resistance contribution in the local rice in South China, followed by Pik-h, Pik-p, Pita, Pii and Piz-t. [Conclusion]This study provided a scientific basis for the rational distribution of resistant varieties with different genotypes in South China.关键词
杂交稻组合/抗病基因/稻瘟病/基因型分析/分子标记Key words
hybrid rice combinations/resistance gene/rice blast/genotype analysis/molecular marker分类
农业科技引用本文复制引用
汪文娟,朱小源,周继勇,汪聪颖,苏菁,封金奇,陈炳,冯爱卿,杨健源,陈深..八个抗稻瘟病基因在华南籼型杂交水稻中的分布[J].中国水稻科学,2017,31(3):299-306,8.基金项目
国家重点研发计划项目(2016YFD0300700) （2016YFD0300700）
广东省科技计划资助项目(2015B020231003 （）
2016B090918116 （）
2016A050502030) （）
国家水稻产业技术体系资助项目(CARS-01-24). （CARS-01-24）
