中国比较医学杂志2017，Vol.27Issue(5)：23-30,8.DOI:10.3969.j.issn.1671-7856.2017.05.008
血小板通过炎症反应在盐敏感性高血压中的作用机制研究
The study on the mechanism of platelet in the development of salt-sensitive hypertension via inflammation
摘要
Abstract
Objective To investigate the role and mechanism of platelet in the development of salt-sensitive hypertension.Methods 25 Dahl salt-sensitive rats (Dahl SS) were divided into three groups: low-salt diet (0.12% NaCl, LS), high-salt diet (8%NaCl, HS) and high-salt diet + platelet inhibitor (8%NaCl+busulfan, HS+bus).Blood pressures were measured by tail-cuff method.After six weeks, animals were sacrificed.Platelet p-selectin expression, platelet cytosolic Ca2+ concentration, platelet-leukocyte aggregation (PLA) in peripheral blood, and immune cells infiltrated on aortic walls were assessed by flow cytometry, and serum IL-6 level was tested by ELISA in vivo.Platelets purified from SD rats were treated with normal salt (0.9%NaCl) and high salt (1.3%NaCl), then the cytosolic Ca2+ concentration and p-selectin expression of platelet were detected.Results We found that Dahl SS rats with high-salt diet, relative to low-salt diet, presented with high blood pressure and increased the ratio of platelet p-selectin expression, Ca2+ concentration.IL-6 level and PLA in peripheral blood, and the number of infiltrated immune cells on aortic walls were also significantly elevated in high-salt diet group.The whole events were ameliorated by the platelet inhibitor busulfan.Cytosolic Ca2+ concentration and p-selectin expression were also increased in purified platelets treated with high salt than those treated with low salt (P < 0.05).Conclusions Our findings suggest that high salt induced platelet activation with increased Ca2+ concentration may play an important role in the development of salt-sensitive hypertension via vascular inflammation.However, the detailed mechanisms of platelet activation and development of high blood pressure via inflammation induced by high salt intake remain to be determined.关键词
盐敏感性高血压/高盐/血小板/炎症Key words
Salt-sensitive hypertension/High salt/Platelet/Inflammation分类
医药卫生引用本文复制引用
毛红亚,刘云鹏,王子皓,姜晓亮,刘星,杨志伟..血小板通过炎症反应在盐敏感性高血压中的作用机制研究[J].中国比较医学杂志,2017,27(5):23-30,8.基金项目
国家自然科学基金面上项目(81600387) （81600387）
国家自然基金青年项目(81600334) （81600334）
研究所基本业务费(DWS201604). （DWS201604）
