中国比较医学杂志2017，Vol.27Issue(5)：31-36,6.DOI:10.3969.j.issn.1671-7856.2017.05.009
Fkbp51基因敲除对小鼠肝脏转录组基因可变剪接的影响
Effects of Fkbp51 gene knockout on the alternative splicing of liver transcriptome in mice
摘要
Abstract
Objective The purpose of this study is to understand the influence of Fkbp51 gene knockout on alternative splicing of mRNA in liver tissues.Methods mRNAs of liver from both Fkbp51 knockout(KO) and wild type(WT) mice were isolated.mRNA expression profiling was performed using RNA-seq reads.The mRNA reads produced from RNA-seq was analysed by TopHat for alternative splicing.Exon skipping and intron retetion were identified according to alignment analyses.Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment were analyzed using online tools DAVID.All genes which had differently expressed alternative splicing between WT and KO were annotated using NCBI database.Results (1) Fkbp51 KO can introduce new mRNA alternative splicing;(2) Fkbp51 KO also cause mRNA alternative splicing expression change;(3) According to GO and KEGG analysis, it was found that the mRNA alternative splicing changes mainly enriched in fat metabolism, immune, bile acid secretion, and PPAR signaling pathway etc.(4) The genes which have different intron retetion events are mainly involved in regulation of actin cytoskeleton organization and amino acids metabolism.Conclusions Fkbp51 gene knockout can cause the change of mRNA alternative splicing, and thereby affect the metabolism of liver in mice.关键词
Fkbp51基因敲除小鼠/肝脏/RNA-seq/可变剪接/内含子保留/外显子跳跃Key words
FKBP51 knockout mice/Liver/RNA-seq/Alternative splicing/Intron retention/Exon skipping分类
医药卫生引用本文复制引用
周志强,杨志伟,雍伟东..Fkbp51基因敲除对小鼠肝脏转录组基因可变剪接的影响[J].中国比较医学杂志,2017,27(5):31-36,6.基金项目
艾滋病和病毒性肝炎传染病重大专项(2014ZX10004002) （2014ZX10004002）
国家自然科学基金(81272273). （81272273）
