中国比较医学杂志2017，Vol.27Issue(5)：37-41,5.DOI:10.3969.j.issn.1671-7856.2017.05.010
狨猴B2m基因沉默位点在细胞水平的验证
Validation of the silencing site of marmoset B2m gene at the cellular level
摘要
Abstract
Objective To screen and determine the effective silencing targets of β2-microglobulin(B2m)gene at the cellular level in marmoset.Methods By homology comparison of the b2m gene in human and the B2m gene in marmoset, choose homology small hairpin RNA(shRNA)sequences targeting marmoset B2m gene were designed, We choose homology small hairpin RNA(shRNA)sequences targeting designed B2m gene to make homology analysis, and insert into lentivirus-based gene silencing constructs FUGW-TDT.The vectors were transfected into HEK293T cells induced by polyethylenimine(PEI).The suppression of B2m mRNA was detected by real-time PCR.Results Two gene-silencing sequences were screened that lied in 290~310 bp and 665~685 bp of the marmoset B2m mRNA, and have statistical significance in the silencing rate:(46.54±7.91)% (P < 0.05) and(83.22±4.37)%(P < 0.0001).Conclusions Two effective silencing target sequences are screened at cellular level, which can be further used in studies on gene silencing in marmoset.关键词
基因沉默/狨猴/β2-微球蛋白/短发夹RNAKey words
Gene silence/Marmoset/B2m/shRNA分类
医药卫生引用本文复制引用
邓怡晨,张晨,向志光,滕永康,刘云波..狨猴B2m基因沉默位点在细胞水平的验证[J].中国比较医学杂志,2017,27(5):37-41,5.基金项目
国家科技支撑计划(2014BAI03B01). （2014BAI03B01）
