中国比较医学杂志2017，Vol.27Issue(5)：42-50,9.DOI:10.3969.j.issn.1671-7856.2017.05.011
Kruppel样因子6对肝癌HepG2细胞的作用及其缺失对斑马鱼肝脏发育的影响
Effects of Kruppel-like factor 6 on HepG2 and the development of liver in zebrafish
摘要
Abstract
Objective To investigate the effects of Kruppel-like factor 6 (KLF6) on the apoptotic and migration ability of HepG2 cell, and the developmental role of KLF6 on zebrafish liver.Methods Constructed plasmid with shRNA-KLF6 was transfected in HepG2 and L-02.The impacts of loss of KLF6 on HepG2 was investigated by Western bolt, apoptosis analyses, cell cycle detection and scratch experiment;KLF6 morpholino oligonucleotides was microinjected into the Tg(lfabp:eGFP) transgenic zebrafish embryos.The morphant phenotype of the liver was imaged and the protein expression of KLF6 after knockdown of KLF6 was analyzed by Immunofluorescence staining.Results The expression of KLF6 in L-02 was significantly higher than in HepG2.After knockout of KLF6, KLF6 protein expression and apoptosis were significantly reduced.In addition, the cell cycle mainly stated in S phase and the migration ability of HepG2 was enhanced.After klf6 knockdown in transgenic zebrafish larvae, the development of zebrafish liver was delayed and KLF6 expression was obviously decreased in the liver.Conclusions The reduction of KLF6 expression increased the proliferation and migration ability, and reduced the apoptosis of HepG2.Loss of KLF6 affects the development of zebrafish liver, which may open a possibility to use zebrafish as a liver cancer model and for anti-liver cancer drug screening.关键词
KLF6基因/HepG2细胞/斑马鱼/MorpholinoKey words
KLF6/HepG2/Zebrafish/Morpholino分类
医药卫生引用本文复制引用
陈浩,杨振国,伍水龙,邬永富,冯瑜菲,高晓霞,包仕廷,张晶晶..Kruppel样因子6对肝癌HepG2细胞的作用及其缺失对斑马鱼肝脏发育的影响[J].中国比较医学杂志,2017,27(5):42-50,9.基金项目
广东省科技计划项目(2013B060300021) （2013B060300021）
广东省"培养高层次人才特殊支持计划"专项基金(粤人才办〔2015〕8号) （粤人才办〔2015〕8号）
广东省高等学校优秀青年教师培养计划(YQ2015081) （YQ2015081）
广东医科大学科研基金(XK1418). （XK1418）
