中国比较医学杂志2017，Vol.27Issue(5)：89-93,5.DOI:10.3969.j.issn.1671-7856.2017.05.019
脱氧紫色杆菌素抑制结肠癌TH29细胞增殖及诱导凋亡作用与机制
Effect of deoxyviolacein on inhibiting proliferation and inducing apoptosis of colon cancer HT29 cells
张海燕 1孙勇 2夏惠 3王锋 4王少康 2邢新会 2孙桂菊 2彭景3
作者信息
- 1. 扬州大学食品科学与工程学院,江苏 扬州 225000
- 2. 东南大学公共卫生学院营养与食品卫生系, 教育部环境医学工程重点实验室,南京 210009
- 3. 清华大学化学工程系,工业生物催化教育部重点实验室, 北京 100084
- 4. 中国肉类食品综合研究中心,北京 100068
- 折叠
摘要
Abstract
Objective To evaluate the effect of deoxyviolacein on the apoptosis in HT29 cells and its possible mechanisms of anticancer effects.Methods In vitro,human colon cancer HT29 cells were treated by deoxyviolacein in a series of concertrations.The absorbance value was detected by means of MTT assay and growth inhibition rate was calculate.Cell cycle and apoptosis was assessed by flow cytometry.The expression of Bax,Bcl-2 and Caspase9 were analyzed by Western blotting.Results Deoxyviolacein could inhibit the growth of human colon cancer HT29 cells.The cells at period G0/G1 and the apoptosis rates were significantly increased,and the cells at period S were significantly decreased.The expression of Bax,Caspase9 were upregulated and Bcl-2 was downregulated by deoxyviolacein at 48 hours.Conclusions Deoxyviolacein could inhibit the growth and induce apoptpsis of human colon cancer HT29 cells.The mechanism may be associated with up-regulation of Bax and Caspase9 and down-regulation of Bcl-2 expression.关键词
脱氧紫色杆菌素/HT29细胞/凋亡Key words
Deoxyviolacein/HT29 cells/Apoptpsis分类
医药卫生引用本文复制引用
张海燕,孙勇,夏惠,王锋,王少康,邢新会,孙桂菊,彭景..脱氧紫色杆菌素抑制结肠癌TH29细胞增殖及诱导凋亡作用与机制[J].中国比较医学杂志,2017,27(5):89-93,5.
