中国动物传染病学报2017，Vol.25Issue(2)：1-8,8.
犬瘟热病毒AH株全基因组序列测定及其分子特征
GENOME SEQUENCING AND ANALYSIS OF CANINE DISTEMPER VIRUS STRAIN AH
摘要
Abstract
The complete genome of Canine distemper virus (CDV) strain AH isolated from Anhui province was subcoloned and sequenced in the present study. Total 8 pairs of specific primers were designed according to the CDV reference for amplification of the complete genome of CDV-AH strain in RT-PCR. Subsequently, the DNAStar software and MEGA software were used to compare and analyze the gene sequences of CDV-AH strain with reference strains. The results showed that total length of CDV-AH was 15690 bp encoding 6 structural proteins (N P, M, F, H and L). The CDV-AH strain had 98.8% nucleotide sequence homology with CDV-PS strain and 92.1% with CDV-1 and Onderstepoort vaccine strains. The analysis of amino acid sequences showed 15 amino acid mutations in the conserved region of CDV-AH and the effect of these mutations on the protein structure and function need to be further studied. In addition, phylogenetic analysis of the CDV-AH strain and reference strains was carried out based on the H gene sequence. The results showed that the CDV-AH strain and other CDV wild strains together came from a topological group belonging to the Asian Ⅰ type.关键词
犬瘟热病毒/全基因组/序列分析Key words
Canine distemper virus/complete genome/sequence analysis分类
农业科技引用本文复制引用
刘柱,李丹丹,李琦,宫晓华,缪秋红,李传峰,陈宗艳,张淼涛,刘光清..犬瘟热病毒AH株全基因组序列测定及其分子特征[J].中国动物传染病学报,2017,25(2):1-8,8.基金项目
国家自然科学基金项目(31270194 , 31502068) （31270194 , 31502068）
上海市科技兴农重点攻关项目(2016043) （2016043）
上海市科委创新项目 (13391901602) （13391901602）
公益性农业科研专项(201303046) （201303046）
中央级公益性科研院所基本科研业务费专项(2016JB01, 31101848) （2016JB01, 31101848）
